Proteomics

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Evolution of the senescence-related proteome in rapeseed roots in response to nitrogen deficiency

ABSTRACT: Senescence related markers have been widely studied in leaves in many plant species. Root senescence is more difficult to characterize. Here we analyse the evolution of rapeseed root proteome during the reproductive stage when monocarpic senescence and grain filling occur. We compare this evolution between two different root compartments, the taproot, which has a storage function and the lateral roots, which have an absorption function. This comparison was carried out in hydroponic culture under nitrogen limiting conditions, which are known to exacerbate senescence and proteolysis in leaves. The shotgun proteomic approach has enabled us to highlight the identification of root senescence marker. This analysis shows two different behaviours between the two root organs and provides a temporal analysis of the change in the proteome. The analysis of protease changes provides a list of candidates that may play a role in nitrogen and carbohydrate remobilization from taproot to the shoot and flowering organs.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Root

SUBMITTER: Thierry Balliau  

LAB HEAD: Dr Philippe Etienne

PROVIDER: PXD050894 | Pride | 2025-05-06

REPOSITORIES: Pride

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Publications

Multi-scale phenotyping of senescence-related changes in roots of rapeseed in response to nitrate limitation.

James Maxence M   Masclaux-Daubresse Céline C   Balliau Thierry T   Marmagne Anne A   Chardon Fabien F   Trouverie Jacques J   Etienne Philippe P  

Journal of experimental botany 20250101 2

Root senescence remains largely unexplored. In this study, the time-course of the morphological, metabolic, and proteomic changes occurring with root aging were investigated, providing a comprehensive picture of the root senescence program. We found novel senescence-related markers for the characterization of the developmental stage of root tissues. The rapeseed root system is unique in that it consists of the taproot and lateral roots. Our study confirmed that the taproot, which transiently acc  ...[more]

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