Long-read Proteogenomics Internal Standard triggered PRM (LRP-IS-PRM)

ABSTRACT: Long-read proteogenomic data was used to create sample-matched protein database for WTC11 sample. This includes many potential alternative protein isoforms (major and minor isoforms) per gene. The IS-PRM method called Tomahto was tested versus DDA to demonstrate improved coverage of isoform-specific peptides. We called this overall method of long-read RNA informed Tomahto targeting LRP-IS-PRM and used it for protein-level evidence of multiple isoforms derived from alternative splicing.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Erin Jeffery  

LAB HEAD: Gloria Sheynkman

PROVIDER: PXD050909 | Pride | 2024-07-17

REPOSITORIES: Pride