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Evaluation of the technical reproducibility of serum proteome sample preparation methods to support clinical proteomics applications

ABSTRACT: Evaluation of the efficiency and reproducibility of sample prepration methods using iST-BCT kit from PreOmics or SMART Trypsin Kit (Thermo Fisher) with in-house Rapigest protocol in human serum samples prior to mass spectrometry analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Jesenia Perez

LAB HEAD: Stefani N. Thomas

PROVIDER: PXD051002 | Pride | 2025-04-07

REPOSITORIES: Pride

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			Action	DRS
	4_B198_SMT_4ug_1.msf	Msf
	4_B198_SMT_4ug_1.pdResult	Other
	4_B198_SMT_4ug_1.raw	Raw
	4_B198_iST_4ug_1.msf	Msf
	4_B198_iST_4ug_1.pdResult	Other

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Publications

Evaluation of Serum Proteome Sample Preparation Methods to Support Clinical Proteomics Applications.

Twigg Carly A I CAI Perez Jesenia M JM Ryu Joohyun J Hanson Benjamin K BK Barrera Estrada Valerie J VJ Thomas Stefani N SN

Journal of the American Society for Mass Spectrometry 20240912 11

Serum contains several proteins that are associated with disease-related processes. Mass spectrometry (MS)-based proteomics approaches greatly facilitate serum protein biomarker development. However, the serum proteome complexity presents a technical challenge for the accurate, sensitive, and reproducible quantification of proteins by MS. Thus, efficient sample preparation methods are of critical importance for serum proteome analyses. In this study, we evaluated the technical performance of two ...[more]

PMID: 39263706

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