Proteomics

Dataset Information

0

Atypical efferocytosis of live neutrophils dysregulates inflammation during P. gingivalis infection

ABSTRACT: Here, we show that neutrophils infected with the bacterial pathogen Porphyromonas gingivalis (Pg) are internalized alive by macrophages in a manner that bypasses all known efferocytic receptor-ligand interactions with highly inflammatory outcomes. Mechanistically, proteolytic cleavage of neutrophil granule proteins by the Pg protease RgpB generated non-canonical ligands that were recognized by macrophage aMb2 (CR3) receptors, facilitating the efferocytosis of live neutrophils. Contrary to the immunosuppression induced by apoptotic cells, efferocytosis of Pg-infected or RgpB-treated live neutrophils was highly inflammatory, with significant delays in efferosomal maturation. Thus, our data show a novel immune subversion strategy employed by a bacterial pathogen that utilizes a previously unknown receptor-ligand interaction for the efferocytosis of live cells and consequently dysregulates the resolution of inflammation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Neutrophil

SUBMITTER: Luiz Gustavo de Almeida  

LAB HEAD: Dr. Antoine Dufour

PROVIDER: PXD051073 | Pride | 2025-07-14

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
S13632_569_Gingapain_PreTails_Day_1_220904.raw Raw
S13633_570_Gingapain_PreTails_Day_3_220904.raw Raw
S13634_571_Gingapain_PreTails_Day_5_220904.raw Raw
S13635_572_Gingapain_PreTails_Day_6_220904.raw Raw
S13636_573_Gingapain_Tails_Day_1_220904.raw Raw
Items per page:
1 - 5 of 10
altmetric image

Publications

Neutrophils are highly abundant in the oral mucosal tissues, and their balanced activation and clearance are essential for immune homeostasis. Here, we demonstrate that neutrophils infected with the bacterial pathogen Porphyromonas gingivalis (Pg) are captured alive by macrophages in a manner that bypasses all known receptor-ligand interactions involved in the phagocytosis of either live or dead cells. Mechanistically, upon interaction with Pg, or its protease RgpB (gingipains), live neutrophils  ...[more]

Similar Datasets

Proteomics Identifying putative substrates of Calpain-15 in neurodevelopment
2025-05-07 | PXD060034 | Pride
Proteomics The Identification of Proteolytic Substrates of Calpain-5 with N-terminomics
2025-07-21 | PXD064313 | Pride
Proteomics Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
2025-03-06 | PXD055867 | Pride
Transcriptomics Erroneous efferocytosis of live neutrophils during infection prevents the resolution of inflammation [RNAseq_dual_species]
2025-08-25 | GSE264523 | GEO
Transcriptomics Erroneous efferocytosis of live neutrophils during infection prevents the resolution of inflammation [RNAseq_single_species]
2025-08-25 | GSE264522 | GEO
Proteomics Tryptase regulation of joint lubrication and inflammation in osteoarthritis
2023-03-04 | PXD037040 | Pride
Proteomics N-terminomics and proteomics analysis of Calpain-2 reveal key proteolytic processing of metabolic and cell adhesion proteins
2025-04-25 | PXD054339 | Pride
Proteomics N-terminomics/TAILS profiling of macrophages after chemical inhibition of legumain
2019-09-05 | PXD015200 | Pride
Genomics Erroneous efferocytosis of live neutrophils during infection prevents the resolution of inflammation
| PRJNA1103064 | ENA
Genomics Erroneous efferocytosis of live neutrophils during infection prevents the resolution of inflammation [RNAseq_dual_species]
| PRJNA1103070 | ENA