Conventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure

ABSTRACT: We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Andrew Michael  

LAB HEAD: David C. Schriemer

PROVIDER: PXD051075 | Pride | 2024-04-12

REPOSITORIES: Pride