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Identification of E2f1 interactors during early embryonic development in Xenopus tropicalis

ABSTRACT: In this project, we performed E2f1 immunoprecipitation followed by mass spectrometry analysis to identify its interacting partners during early embryonic development in Xenopus tropicalis.

INSTRUMENT(S):

ORGANISM(S): Xenopus Tropicalis

TISSUE(S): Embryo

SUBMITTER: Huanhuan Cui

LAB HEAD: Wei Chen

PROVIDER: PXD051084 | Pride | 2025-12-01

REPOSITORIES: Pride

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	E2F1-1.raw	Raw
	E2F1-2.raw	Raw
	checksum.txt	Txt
	ctrl-1.raw	Raw
	ctrl-2.raw	Raw

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Publications

CANTAC-seq analysis reveals E2f1 and Otx1 coordinate zygotic genome activation in Xenopus tropicalis.

Cui Huanhuan H Liang Weizheng W Shi Zhaoying Z Zhang Luming L Li Guipeng G Chen Rui R Tian Chi C Gan Diwen D Shi Xinyao X Sun Zhiyuan Z Zhu Qionghua Q Fang Liang L Huang Hongda H Hu Yuhui Y Chen Yonglong Y Chen Wei W

Nature communications 20251118 1

Zygotic genome activation is tightly associated with the modulation of chromatin accessibility via maternal transcription factors. Understanding how chromatin accessibility is established and identifying key maternal regulators are crucial to comprehending this process. Here, by developing CANTAC-seq, we generate a genome-wide map of accessible chromatin of early Xenopus tropicalis embryos and find that the open chromatin landscape is progressively established at cis-regulatory elements during z ...[more]

PMID: 41253814

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Project description:In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.

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