Proteomics

Dataset Information

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Investigating the impact of GalNAc-T2 overexpression on proteomics and glycoproteomics in lung cell

ABSTRACT: GalNAc-T2 was identified as a novel antiviral factor that promotes viral clearance and contributes to reduced disease severity.This project aimed to investigate the impact of GalNAc-T2 overexpression on proteomics and glycoproteomics in lung cell. We employ SILAC labeling, lectin-based affinity enrichment, and LC-ETD MS/MS to characterize the regulated proteins and glycosylation sites resulting from GalNAc-T2 overexpression.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Yuqi He  

LAB HEAD: Yang Mao

PROVIDER: PXD051101 | Pride | 2026-01-12

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20220425_HYQ_Spike_WT_PNG_trypsin_chymo_Neu.raw Raw
20240103_HYQ_A549_SILAC_1.raw Raw
20240103_HYQ_A549_SILAC_2.raw Raw
20240103_HYQ_A549_SILAC_3.raw Raw
20240103_HYQ_A549_SILAC_quantification.pdResult Other
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Publications

Interferon-stimulated gene GALNT2 restricts respiratory virus infections.

Ran Wei W   Yang Jinghong J   Yu Shi S   Hu Qingtao Q   He Yuqi Y   Wang Shengjun S   Wei Cheng C   Sun Jing J   Zhu Airu A   Li Fang F   Zhang Lu L   Li Pengfei P   Hu Huina H   Pan Ruangang R   Yu Yanying Y   Yuan Yanqiu Y   Sun Lingyu L   Wang Yanqun Y   Zhang Zhaoyong Z   Chen Zhao Z   Liu Donglan D   Yan Qihong Q   Wang Dong D   Yu Kuai K   Wang Yiliang Y   Zhuo Jianfen J   Zhuang Zhen Z   Tao Hua H   Zeng Qiuhui Q   Zhou Kaixin K   Chen Fenghua F   Tang Wenxuan W   Zhou Yuzheng Y   Bai Rong R   Xu Yonghao Y   Li Na N   Dai Jun J   Zhao Hongyu H   Park Jung-Eun JE   Wang Tao T   Ding Qiang Q   Perlman Stanley S   Zhao Jingxian J   Mao Yang Y   Wang Shaobo S   Zhao Jincun J  

Nature microbiology 20251212 1

The innate immune response involves interferons (IFNs), antiviral cytokines that upregulate numerous IFN-stimulated genes, many of which have uncharacterized functions and mechanisms. Here we performed transcriptomic profiling of lung tissues from wild-type and IFNAR<sup>-/-</sup> mice infected with SARS-CoV-2 and single-cell RNA sequencing of bronchoalveolar lavage fluid and peripheral blood mononuclear cells from patients with COVID-19. We identified O-GalNAc transferase 2 (GALNT2), an N-acety  ...[more]

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