Dataset Information

SCAMP3 is a component of insulin granules in MIN6 -cells and regulates insulin secretion and content

ABSTRACT: Pancreatic -cells in the islets of Langerhans are key to maintaining glucose homeostasis, by secreting the peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), that have recently been considered to have intrinsic structures and proteins that regulate insulin granule maturation, trafficking, and secretion. Previously, studies have identified a handful of novel ISG-associated proteins using different separation techniques. Here, this study combines an optimized ISG isolation technique and mass spectrometry-based proteomics, with an unbiased and targeted machine learning approach to uncover 211 ISG-associated proteins. Five of these proteins have not been previously ISG-associated: Syntaxin-7, Synaptophysin, Synaptotagmin-13, Zinc transporter ZIP8 and Scamp3. We validate the role for one of these novel ISG-associated proteins (Scamp3) in regulating insulin storage and secretion from -cells for the first time. These data provide the basis for future investigation into beta cell biology and the regulation of insulin secretion.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Mark Larance

LAB HEAD: Mark Larance

PROVIDER: PXD051136 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210609_Eclipse_ML_NN_EXP1_SAMPLEA_FRAC1.raw	Raw
	20210609_Eclipse_ML_NN_EXP1_SAMPLEA_FRAC10.raw	Raw
	20210609_Eclipse_ML_NN_EXP1_SAMPLEA_FRAC11.raw	Raw
	20210609_Eclipse_ML_NN_EXP1_SAMPLEA_FRAC12.raw	Raw
	20210609_Eclipse_ML_NN_EXP1_SAMPLEA_FRAC2.raw	Raw

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Publications

Optimized Proteomic Analysis of Insulin Granules From MIN6 Cells Identifies Scamp3, a Novel Regulator of Insulin Secretion and Content.

Norris Nicholas N Yau Belinda B Famularo Carlo C Webster Hayley H Loudovaris Thomas T Thomas Helen E HE Larance Mark M Senior Alistair M AM Kebede Melkam A MA

Diabetes 20241201 12

Pancreatic β-cells in the islets of Langerhans are key to maintaining glucose homeostasis by secreting the peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), which recently have been considered to have intrinsic structures and proteins that regulate insulin granule maturation, trafficking, and secretion. Previously, studies have identified a handful of novel ISG-associated proteins, using different separation techniques. The present study combin ...[more]

PMID: 39320956

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