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Deciphering hepatitis B virus phospho-signature in primary human hepatocytes

ABSTRACT: Phosphorylation is a major post-translation modification (PTM) of proteins, and small molecules, which is finely tuned by the activity of several hundred kinases and phosphatases. It controls most if not all cellular pathways including anti-viral responses. Accordingly, viruses often induce important changes in the phosphorylation of host factors that can either help or counteract viral replication. Surprisingly, among more than 500 kinases constituting the human kinome only few have been described as important for the Hepatitis B virus (HBV) infectious cycle, and most of them intervene during early or late infectious steps by phosphorylating the viral Core protein (HBc) protein. In addition, scarce information is available on the consequences of HBV infection on the activity of cellular kinases. The objective of this study was to investigate the global impact of HBV infection on the cellular phosphorylation landscape at early after infection. For this, primary human hepatocytes (PHH) were challenged or not with HBV, and a mass spectrometry (MS)-based quantitative phospho-proteomic analysis was conducted two- and seven-days post-infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Hepatitis B Virus Subtype Ayw

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD051216 | Pride | 2024-05-02

REPOSITORIES: Pride

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