Dataset Information

Factors of metronidazole resistance in the human parasite Trichomonas vaginalis

ABSTRACT: Trichomonas vaginalis is a sexually transmitted human parasite causing trichomoniasis, leading to vaginitis or cervicitis in many patients. Furthermore, infections can lead to premature labour or loss of child as well as an increase in the risk of contracting HIV. For treatment the antibiotic 5-nitroimidazole metronidazole is commonly used, however antibiotic resistance in TV is on the rise, and the mechanism of how the parasite becomes resistant is not yet known. The aim of the study thus was to unravel the mechanism of resistance formation, by understanding the change in protein expression of the parasite. For this we studied different clinical isolates (i.e. various strains of TV) that were either sensitive or resistant to metronidazole, or resistance has been induced in a lab strain. It was previously known, that when sensitive TV cells are depleted of iron, they appear to have the same characteristics as resistant cell lines, however remained sensitive to the drug. Several proteins were identified, where the protein expression differed between the same cell line where either cells were depleted of iron, or resistance was induced. These proteins (mainly reductive agents) were studied on more detail. To understand the mechanism of metronidazole resistance.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trichomonas Vaginalis

TISSUE(S): Cell Culture

SUBMITTER: Anna-Lena Mayr

LAB HEAD: Ebrahim Razzazi-Fazeli

PROVIDER: PXD051345 | Pride | 2025-03-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2302032_TVC1_1_1_Irondepletion_A.msf	Msf
	2302032_TVC1_1_1_Irondepletion_A.mzML	Mzml
	2302032_TVC1_1_1_Irondepletion_A.raw	Raw
	2302032_TVC1_1_1_Irondepletion_B.msf	Msf
	2302032_TVC1_1_1_Irondepletion_B.mzML	Mzml

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Publications

Comparative proteomic analysis of metronidazole-sensitive and resistant Trichomonas vaginalis suggests a novel mode of metronidazole action and resistance.

Mayr Anna-Lena AL Paunkov Ana A Hummel Karin K Razzazi-Fazeli Ebrahim E Leitsch David D

International journal for parasitology. Drugs and drug resistance 20240926

The microaerophilic parasite Trichomonas vaginalis occurs worldwide and causes inflammation of the urogenital tract, especially in women. With 156 million infections annually, trichomoniasis is the most prevalent non-viral sexually transmitted disease. Trichomoniasis is treated with 5-nitroimidazoles, especially metronidazole, which are prodrugs that have to be reduced at their nitro group to be activated. Resistance rates to metronidazole have remained comparably low, but they can be higher in ...[more]

PMID: 39368438

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