FTO inhibition attenuates obstruction-induced kidney fibrosis by downregulating ferroptosis activator ACSL4 and profibrotic factor TGFBI

ABSTRACT: Analysis by LC-MS/MS shotgun proteomics was performed on lysates from the following samples: 1) Control HK2 cells generated using control CRISPR/CAS9 system and scrambled guide RNA (sgRNAGCACTCACATCGCTACATCA) 2) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 2 guide RNA (sgCCTACAAGTACCTGAACACC) 3) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 3 guide RNA (sgCCAGGCTCTTTACGGTCCCC) 4) HK2 cells treated with vehicle control 5) HK2 cells treated with erastin 6) HK2 cells treated with FTO inhibitor FB23-2

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Proximal Tubule

SUBMITTER: Sharon Pitteri  

LAB HEAD: Sharon Pitteri

PROVIDER: PXD051362 | Pride | 2025-10-13

REPOSITORIES: Pride