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Proteome of fission yeast RNA degradation mutants

ABSTRACT: We measured total proteomes of 15 different fission yeast strains : wild type, Δcid1, Δdis32, Δcid1Δdis32, Δlsm1, Δski2, Δcid1Δlsm1, Δdis32Δski2, Δcid1Δski2, Δcid1Δdis32Δski2, Δcid1Δdis32Δlsm1, Δdis32Δlsm1, Δlsm1Δski2, Δlsm1Δski2Δdis32, Δlsm1Δski2Δcid1. All mutantions affected cytoplasmic mRNA decay. At least 5 repeats were measured for each mutant.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe 927 Schizosaccharomyces Pombe

SUBMITTER: Anja Freiwald

LAB HEAD: Michal Malecki

PROVIDER: PXD051392 | Pride | 2024-06-21

REPOSITORIES: Pride

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			Action	DRS
	20220502_Z2_KTT_001_30-0055_RnaDeg_P01_B01.wiff2.Zip	Wiff
	20220502_Z2_KTT_002_30-0055_RnaDeg_P01_C01.wiff2.Zip	Wiff
	20220502_Z2_KTT_003_30-0055_RnaDeg_P01_F01.wiff2.Zip	Wiff
	20220502_Z2_KTT_004_30-0055_RnaDeg_P01_G01.wiff2.Zip	Wiff
	20220502_Z2_KTT_005_30-0055_RnaDeg_P01_H01.wiff2.Zip	Wiff

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Publications

Uridylation regulates mRNA decay directionality in fission yeast.

Grochowski Maciej M Lipińska-Zubrycka Lidia L Townsend StJohn S Golisz-Mocydlarz Anna A Zakrzewska-Płaczek Monika M Brzyżek Grzegorz G Jurković Borna B Świeżewski Szymon S Ralser Markus M Małecki Michał M

Nature communications 20240927 1

Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5' to 3' or 3' to 5' direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3'-RACE (gw3'-RACE) in the model system fission yeast to elucidate the role of uridylation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5' to 3' mR ...[more]

PMID: 39333464

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