Biotinylated MMH2 pulldown LCMS

ABSTRACT: Here, we present structural and mechanistic studies that define a trans-labeling covalent molecular glue mechanism. We found that a novel series of BRD4 molecular glue degraders act by recruiting the CUL4-DCAF16 ligase to the second bromodomain of BRD4. To gain a deeper understanding into the potential off-target effects of more reactive degraders, we conducted streptavidin pulldowns of K562 lysate incubated with engineered MMH2-Biotin, a probe molecule that appends linker-biotin onto the second exit vector of MMH2.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Anna Schmoker  

LAB HEAD: Eric Fischer

PROVIDER: PXD051457 | Pride | 2025-05-06

REPOSITORIES: Pride