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Chemical crosslinking of photosystem I antenna proteins

ABSTRACT: Chemical crosslinking (DSS) of proteins in conjunction with mass spectrometry was performed to identify protein-protein interactions between the light-harvesting proteins of PSI and PSII.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Martin Scholz

LAB HEAD: Michael Hippler

PROVIDER: PXD051493 | Pride | 2025-04-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Combined_results.xlsx	Xlsx
	HX_Crosslinks-27052014-NEU-noMT_4-1.mgf	Mgf
	HX_Crosslinks-27052014-NEU-noMT_4-1.raw	Raw
	HX_Crosslinks-27052014-NEU-noMT_4-10.mgf	Mgf
	HX_Crosslinks-27052014-NEU-noMT_4-10.raw	Raw

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Publications

Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 in <i>Chlamydomonas reinhardtii</i>.

Mosebach Laura L Ozawa Shin-Ichiro SI Younas Muhammad M Xue Huidan H Scholz Martin M Takahashi Yuichiro Y Hippler Michael M

Plants (Basel, Switzerland) 20240613 12

The photosystem I (PSI) of the green alga <i>Chlamydomonas reinhardtii</i> associates with 10 light-harvesting proteins (LHCIs) to form the PSI-LHCI complex. In the context of state transitions, two LHCII trimers bind to the PSAL, PSAH and PSAO side of PSI to produce the PSI-LHCI-LHCII complex. In this work, we took advantage of chemical crosslinking of proteins in conjunction with mass spectrometry to identify protein-protein interactions between the light-harvesting proteins of PSI and PSII. W ...[more]

PMID: 38931064

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