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Two-photon polymerization of miniaturised 3D scaffolds optimised for studies on glioblastoma multiforme in spaceflight-like microgravity conditions

ABSTRACT: The obtainment of innovative models recapitulating the complex tumor architecture and activity in vitro is a challenging drive in the understanding of pathology molecular bases, yet a crucial passage to the identification of targets for advanced oncotherapy. Topography tuning and gravitational unloading of cell culture can represent useful means in tumor biomimicry process: in this work, both were chosen for modelling of glioblastoma multiforme, a highly aggressive neoplasm that affects the central nervous system and that has poorly effective pharmacological and radiological countermeasures. Tumor modeling was pursued by the application of two-photon polymerization to fast prototyping of scaffolds suitable for U87-MG glioma cell culture, and by the exposure of cell-scaffold hybrids to simulated microgravity (s-µg) by rotation on random positioning machine. Glioma cell response was investigated by microscopy, quantitative reverse transcription-polymerase chain reaction and proteomic analyses, denoting synergic regulatory effects of cell scaffolding and s-µg on markers of tumor cell growth, metabolism and invasiveness.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Martina Bartolucci

LAB HEAD: Andrea Petreto

PROVIDER: PXD051522 | Pride | 2025-02-27

REPOSITORIES: Pride

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			Action	DRS
	20221124_114607_Ciofani.sne	Other
	Ciofani_Genchi_1122_Flat_0g_060922_62.raw	Raw
	Ciofani_Genchi_1122_Flat_0g_130922_63.raw	Raw
	Ciofani_Genchi_1122_Flat_0g_220922_64.raw	Raw
	Ciofani_Genchi_1122_Flat_1g_060922_58.raw	Raw

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