Proteomics

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Mass spectrometry of plasma extracellular vesicles before and after exercise

ABSTRACT: We aimed to identify changes in the proteome of extracellular vesicles released into circulation by 7 recreationally active endurance running-trained healthy females after a bout of 60-min incremental treadmill exercise. Blood samples were collected immediately before and after exercise and extracellular vesicles were isolated from plasma by size exclusion chromatography. The samples were then analyzed by mass spectrometry proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Manuel Ramirez  

LAB HEAD: Alicia Llorente Martinez

PROVIDER: PXD051559 | Pride | 2026-02-09

REPOSITORIES: pride

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MR_230120_Llorente_10_Slot2-10_1_7818.mgf Mgf
MR_230120_Llorente_10_Slot2-10_1_7818.mzxml Mzxml
MR_230120_Llorente_11_Slot2-11_1_7820.mgf Mgf
MR_230120_Llorente_11_Slot2-11_1_7820.mzxml Mzxml
MR_230120_Llorente_12_Slot2-12_1_7822.mgf Mgf
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Publications

The Redox Enzyme Thioredoxin Is Increased in Plasma Extracellular Vesicles From Endurance-Trained Females in Response to Acute Exercise.

Sagini Krizia K   Selvarajah Mughilan M   Romero Silvana S   Bassols-Citores Eva E   Martin-Gracia Beatriz B   Ramirez-Garrastacho Manuel M   Rise Fabian F   Endzeliņš Edgars E   Sadovska Lilite L   Skorinkina Diāna D   Boguševiča Marija M   Cešeiko Rudolfs R   Čampa Mārtiņš M   Klavina Aija A   Linē Aija A   Llorente Alicia A  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20250801 16

The effects of exercise are mediated by molecules secreted into the circulation in response to exercise. Extracellular vesicles (EVs) have recently appeared as possible vehicles for some of these molecules, and as mediators of tissue crosstalk during exercise. This study aimed to identify changes in the proteome of EVs released into circulation by endurance running-trained females after a bout of 60-min aerobic exercise consisting of incremental treadmill running. Blood samples were collected im  ...[more]

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