Proteomics

Dataset Information

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Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to the extracellular fluid proteome


ABSTRACT: The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservation. The proteomic changes in turkey (Meleagris gallopavo) semen throughout the cryopreservation process have never been investigated previously. Therefore, the purpose of the present study was to evaluate the proteomic changes of extracellular fluid (ECF)of semen during cryopreservation at three stages (fresh, equilibrated, and frozen/thawed semen) using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF/TOF). Six protein spots were significantly enriched in ECF proteome after equilibration compared to fresh semen. Twenty seven protein spots were enriched in ECF after cryopreservation compared to fresh semen. Twenty six protein spots were significantly enriched in ECF proteome after cryopreservation compared to equilibrated semen. The proteins enriched in ECF after cryopreservation were mainly related to NADH metabolic and glycolytic process.

INSTRUMENT(S): autoflex

ORGANISM(S): Meleagris Gallopavo (common Turkey)

TISSUE(S): Seminal Plasma

SUBMITTER: Mariola Słowińska  

LAB HEAD: Mariola Słowińska

PROVIDER: PXD051615 | Pride | 2025-07-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
checksum.txt Txt
spot_1001.dat Other
spot_1001.mgf Mgf
spot_1001.zip Other
spot_1028.dat Other
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Publications


Two-dimensional difference in-gel electrophoresis coupled with matrix-assisted laser desorption/ionization mass spectrometry was employed to investigate the proteome alterations induced by equilibration and freezing/thawing processes, both in turkey spermatozoa and in extracellular fluid (ECF). The freezing/thawing process resulted in reduced semen quality parameters. Viability and motility decreased threefold (90.6 %-31.2 % and 76.0 %-26.7 %, respectively), while the proportion of live spermato  ...[more]

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