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Label-free DDA proteome for identifying the interacting proteins of HBO1

ABSTRACT: HBO1 is an important transcriptional activator for organism to respond to natural stress. Here, we performed IP-MS for determining the interacting proteins of HBO1 under stimulation of BHB.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervical Epithelium, Cervix Epithelium

DISEASE(S): Cervical Cancer

SUBMITTER: siyu wang

LAB HEAD: Kai Zhang

PROVIDER: PXD051692 | Pride | 2025-07-04

REPOSITORIES: Pride

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			Action	DRS
	HBO1-1.raw	Raw
	HBO1-1.xlsx	Xlsx
	HBO1-2.raw	Raw
	HBO1-3.raw	Raw
	checksum.txt	Txt

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SILAC based quantitative 3-hydroxybutyromics of HBO1-WT and HBO1-KO HeLa cells

Project description:Ketogenesis derived BHB facilitates 3-hydroxybutyrylation on lysine linking metabolism with epigenetic regulation. Herein, we found HBO1 served as 3-hydroxybutyryltransferase preferentially catalyzing lysine 9 of histone H3 (H3K9bhb). Moreover, total 1171 Kbhb sites on 582 proteins were identified. Collectively, we firstly descibed HBO1 mediated Kbhb regulatory network in HeLa cells.

2025-07-04 | PXD051668 | Pride

Effects of the JADE-HBO1 complex on gene expression

Project description:We find that 499 genes are up-regulated and 457 are down-regulated in response to over-expression of JADE1, while 397 genes are up-regulated and 385 are down-regulated after HBO1 knock-down. For each condition - HBO1 siRNA treatment or JADE1 over-expression - two biological replicates were analyzed in duplicate.

2012-03-15 | E-GEOD-33220 | biostudies-arrayexpress

H3K9bhb identification of CD8+Tm cells

Project description:MS/MS spectra of a tryptic H3K9 peptide identified from BHB-treated CD8+ Tm cells or 13C-labled BHB-treated memory T cells

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Genome-wide map of HBO1 in cancer derived human cell line

Project description:We used ChIP-seq technology in order to map chromatin binding sites of the HBO1 MYST complex in the RKO cell line. We obtained a significant enrichment of the HBO1 signal right after TSS regions of genes and also In the proximal promoter region, with no signal on TSS. This enrichment also correlates with gene expression level. HBO1 signal in RKO cell line.

2012-03-15 | E-GEOD-33007 | biostudies-arrayexpress

NUP98-HBO1-induced gene signature in human CB CD34+ cells

Project description:Chronic myelomonocytic leukemia (CMML) is an incurable hematopoietic stem cell malignancy. We identified a novel NUP98-HBO1 fusion from a patient with CMML. HBO1, a histone acetyltransferase (HAT) which belongs to the MYST family, is the first NUP98 fusion partner encodes HAT. To determine the effect of the NUP98-HBO1 fusion on downstream target gene regulation, we performed gene expression array analysis of NUP98-HBO1-transduced human cord blood (CB) CD34+ cells.

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HBO1 Catalyzes Lysine Benzoylation in Mammalian Cells

Project description:Lysine benzoylation (Kbz) is a newly discovered protein post-translational modification (PTM). This PTM can be stimulated by benzoate and contributes to gene expression. However, its regulatory enzymes and substrate proteins remain largely unknown, hindering further functional studies. Here we identified and validated the lysine acetyltransferase (KAT) HBO1 as a “writer” of Kbz in mammalian cells. In addition, we report the first benzoylome in mammalian cells, identifying 1747 Kbz sites, among them at least 77 are the HBO1-targeted Kbz substrates. Bioinformatics analysis showed that HBO1-targeted Kbz sites were involved in multiple processes, including chromatin remodeling, transcription regulation, immune regulation, and tumor growth. Our results thus identify key regulatory elements of the Kbz pathway, and reveal new enzymatic activity and functions of HBO1 in cellular physiology.

2022-10-14 | PXD034187 | Pride

Mapping BRD1 and HBO1 genomic binding in BRCA1 mutant cells

Project description:The BRCA1 tumor suppressor has many functions in DNA damage repair, DNA replication, and beyond. Loss of any of these functions can lead to breast and ovarian carcinogenesis and also therapeutic sensitivity. In this study, we have discovered a novel role for BRCA1 in controlling replication initiation by regulating the bromodomain-protein BRD1 and the histone acetyltransferase HBO1. Specifically, we show that BRCA1 regulates localization of the BRD1-HBO1 complex to replication origins where the complex normally acetylates histone H3 on lysine 14. We performed chromatin-immunoprecipitation seqeuncing (ChIP-seq) for BRD1 and HBO1 in a BRCA1 mutant line (UWB1.289) and demonstrate that BRD1 and HBO1 co-occupy ORC2 binding sites and replication origins.

2024-01-11 | GSE244027 | GEO

HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription

Project description:Lysine lactylation (Kla) links metabolism and gene regulation and plays a key role in multiple biological processes. However, the regulatory mechanism and functional consequence of Kla remain to be explored. Here, we report that HBO1 functions as a lysine lactyltransferase to regulate transcription. Interestingly, CUT&Tag assays demonstrate that HBO1 is required for histone H3K9la on TSSs and the regulated Kla can facilitate in signaling pathways and progress of tumorigenesis. Our study reveals HBO1 serves as a lactyltransferase to mediate a histone Kla-dependent gene transcription.

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Project description:In this study, we have used inducible and tissue-specific genetic deletion to investigate the function of HBO1 in the hematopoietic system. RNA-seq was used to examine the dependence of gene expression on the presence of HBO1(KAT7). Two different conditional expression sytems were used to induce Cre recombinase and delete a floxed allele of HBO1 in this study. This was to control for the treatement effect during Cre induction (interferon induction of Mx1-cre vs. tamoxifen induction of Rosa26-CreERT2). This data set indicates that HBO1 promotes expression of multiple genes encoding a hematopoietic transcription factor network essential for HSC quiescence and self-renewal in adult hematopoiesis.

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SILAC experiment quantifying the proteomics of WT and HBO1-KO HeLa

Project description:Lysine lactylation (Kla) links metabolism and gene regulation and plays a key role in multiple biological processes. We report that HBO1 functions as a lysine lactyltransferase to regulate transcription. Quantitative proteomic analysis further revealsed 95 endogenous Kla sites targeted by HBO1, with the majority located on histones.

2024-04-13 | PXD051415 | Pride