Proteomics

Dataset Information

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Proteomics of mature Bacillus subtilis spores in wild-type and mdfA deletion strains

ABSTRACT: Endospore formation is a starvation-induced developmental pathway in Bacillus subtilis involving an asymmetrically-positioned cell division event that generates two cells, the smaller forespore and the larger mother cell. We previously demonstrated that sporulation is accompanied by metabolic differentiation of these two cells, and the depletion of enzymes in central metabolism from the developing spore. This disables spore metabolism and makes the forespore dependent on mother cell metabolism to produce metabolic building blocks for the biosynthesis of proteins, RNA and phospholipids. The mdfA (yjbA) gene was identified as being required for metabolic differentiation and this data set compares the proteome of wild-type and mdfA spores to assess the impact of metabolic differentiation on metabolism.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacillus Subtilis Py79

SUBMITTER: Jelani Lyda  

LAB HEAD: Kit Pogliano

PROVIDER: PXD051727 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
ER_TMT4_01.mgf Mgf
ER_TMT4_01.raw Raw
ER_TMT4_02.mgf Mgf
ER_TMT4_02.raw Raw
ER_TMT4_03.mgf Mgf
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Publications

Developmentally regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation during <i>Bacillus subtilis</i> sporulation.

Riley Eammon P EP   Lyda Jelani A JA   Reyes-Matte Octavio O   Sugie Joseph J   Kasu Iqra R IR   Enustun Eray E   Armbruster Emily G EG   Ravishankar Sumedha S   Isaacson Rivka L RL   Camp Amy H AH   Lopez-Garrido Javier J   Pogliano Kit K  

Genes & development 20250314

<i>Bacillus subtilis</i> sporulation entails a dramatic transformation of the two cells required to assemble a dormant spore, with the larger mother cell engulfing the smaller forespore to produce the "cell within a cell" structure that is a hallmark of endospore formation. Sporulation also entails metabolic differentiation, whereby key metabolic enzymes are depleted from the forespore but maintained in the mother cell. This reduces the metabolic potential of the forespore, which becomes depende  ...[more]

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