Dataset Information

Biochemical basis of Exo70 specialization

ABSTRACT: Molecular machines comprised by multiple subunits govern essential processes in the cell. Understanding how these complexes evolve is crucial to comprehend the evolution of cellular complexity. However, our understanding on the molecular mechanisms that allow multimeric complexes to change and acquire new functions is still limited. Here we used the plant exocyst complex as a model to study the evolution and mechanistic basis of neo-functionalization in multimeric protein complexes. By leveraging a combination of cell biology, proteomics, biochemistry, and phylogenetic analysis, we show the N-terminal domain of a single exocyst subunit, Exo70, have reverted its electrostatic charge over the course of evolution. This rendered a loose interaction with the rest of the complex and allowed Exo70 to rapidly diversify and acquire novel functions in plants. Our finding shows a novel model in which a single subunit scape from the evolutionary constrains of being in a complex, resulting in high diversification rates and neo-functionalization.

INSTRUMENT(S):

ORGANISM(S): Marchantia Polymorpha Subsp. Ruderalis

TISSUE(S): Plant

SUBMITTER: Richard Imre

LAB HEAD: Yasin F. Dagdas

PROVIDER: PXD051853 | Pride | 2025-12-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20230508_E1_RSLC10_DeLaConcepcion_Dagdas_GMI_ID1161_onbead_5perc_18runs_Step2_Limma_Marchantia_ruderalis_repeat_untilSpace_protchem10.pdResult	Other
	20230508_E1_RSLC10_DeLaConcepcion_Dagdas_GMI_ID1161_onbead_5perc_EV_GFP_01.raw	Raw
	20230508_E1_RSLC10_DeLaConcepcion_Dagdas_GMI_ID1161_onbead_5perc_EV_GFP_02.raw	Raw
	20230508_E1_RSLC10_DeLaConcepcion_Dagdas_GMI_ID1161_onbead_5perc_EV_GFP_03.raw	Raw
	20230508_E1_RSLC10_DeLaConcepcion_Dagdas_GMI_ID1161_onbead_5perc_MpExo70III_GFP_10.raw	Raw

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Publications

Electrostatic changes enabled the diversification of an exocyst subunit via protein complex escape.

De la Concepcion Juan Carlos JC Duverge Héloïse H Kim Yoonwoo Y Julian Jose J Xu Haonan D HD Watt Matthew N MN Ikene Sara Ait SA Bianchi Anita A Grujic Nenad N Papareddy Ranjith K RK Grishkovskaya Irina I Haselbach David D Murray David H DH Clavel Marion M Irwin Nicholas A T NAT Dagdas Yasin Y

Nature plants 20251031 11

Protein neofunctionalization is a key driver of cellular complexity. However, subunits of multimeric protein complexes are often thought to be evolutionarily constrained, limiting their capacity for functional divergence. This presents a paradox in plants, where the Exo70 subunit of the exocyst-an octameric complex essential for exocytosis-has undergone striking expansion and diversification. Here we show that electrostatic changes in the N-terminal helix of Exo70 facilitated its physical and fu ...[more]

PMID: 41174228

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