K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors

ABSTRACT: Ubiquitin-interactor co-pulldown coupled with mass spectrometry used to elucidate K48- and K63-linked interactomes including those of Ub chains of varying length (Ub-Ub3) and heterotypic branched Ub chains. Ubiquitin-interactors were enriched from human HeLa and yeast s. cerevisiae cell lysate with either chloroacetamide (CAA) or N-ethylmaleimide (NEM) as DUB inhibitors.

INSTRUMENT(S):

ORGANISM(S): Candida Albicans

SUBMITTER: Oliver Popp  

LAB HEAD: Thomas Sommer

PROVIDER: PXD051890 | Pride | 2024-05-15

REPOSITORIES: Pride