Proteomics

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MCF7 proteome of cells and EVs during starvation

ABSTRACT: Serum deprivation during cell culture is common in EV research in oder to omit the introduction of xeno-EV material and reduce the presence of contaminating non-EV proteins from bovine serum. However, starvation has a profound effect on the cell proteome. Here, we provide proteome data that shows large differences on the cellular proteome of starved cells and how this is reflected in secreted EV particles of the corresponding cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Breast Cancer Cell

DISEASE(S): Breast Cancer

SUBMITTER: Jarne Pauwels  

LAB HEAD: Kris Gevaert

PROVIDER: PXD051955 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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Action DRS
F006948b_1p_110uPACG2v2_1_trapPM1_CMB-1022_JP-EV-Jana-DIA-FBS0-rep1_1.raw Raw
F006950b_1p_110uPACG2v2_1_trapPM1_CMB-1022_JP-EV-Jana-DIA-FBS2-rep1_2.raw Raw
F006952b_1p_110uPACG2v2_1_trapPM1_CMB-1022_JP-EV-Jana-DIA-FBS5-rep1_3.raw Raw
F006954b_1p_110uPACG2v2_1_trapPM1_CMB-1022_JP-EV-Jana-DIA-FBS10-rep1_4.raw Raw
F006962_1p_110uPACG2v2_1_trapPM1_CMB-1022_JP-EV-Jana-DIA-FBS0-rep2_5.raw Raw
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Publications

Filter-Aided Extracellular Vesicle Enrichment (FAEVEr) for Proteomics.

Pauwels Jarne J   Van de Steene Tessa T   Van de Velde Jana J   De Muyer Freya F   De Pauw Danaë D   Baeke Femke F   Eyckerman Sven S   Gevaert Kris K  

Molecular & cellular proteomics : MCP 20250121 2

Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease pathology. The latter in particular has led to an exponential increase in interest in EVs as they are considered to be circulating packages containing potential biomarkers and are also a possible biological means to deliver drugs in a cell-specific manner. Ho  ...[more]

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