Dataset Information

Comparison of the MCF7 proteome cultured in standard FBS and EV depleted FBS

ABSTRACT: COmparison of the MCF7 proteomes after being cultured for 3 d in either 10% FBS (standard, commercial) or in 10% EV-depleted FBS (EDS, commercial) to validate the absence of EV specific proteins in EDS to exclude potential cross-contamination of xeno-EVs

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Cell, Blood Serum

SUBMITTER: Jarne Pauwels

LAB HEAD: Kris Gevaert

PROVIDER: PXD051956 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20230109_Bovine_rev_SP_6035seq.fasta	Fasta
	FBS_vs_EDS.tsv	Tabular
	H095731_1p_Ionopticks7_PM7_CMB-1405_KRGEV_9.raw	Raw
	H095733_1p_Ionopticks7_PM7_CMB-1405_KRGEV_7.raw	Raw
	H095737_1p_Ionopticks7_PM7_CMB-1405_KRGEV_1.raw	Raw

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Publications

Filter-Aided Extracellular Vesicle Enrichment (FAEVEr) for Proteomics.

Pauwels Jarne J Van de Steene Tessa T Van de Velde Jana J De Muyer Freya F De Pauw Danaë D Baeke Femke F Eyckerman Sven S Gevaert Kris K

Molecular & cellular proteomics : MCP 20250121 2

Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease pathology. The latter in particular has led to an exponential increase in interest in EVs as they are considered to be circulating packages containing potential biomarkers and are also a possible biological means to deliver drugs in a cell-specific manner. Ho ...[more]

PMID: 39842778

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