Proteomics

Dataset Information

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Human CVL LC-MSMS to Elucidate Functions in Bacterial Vaginosis


ABSTRACT: Bacterial vaginosis (BV) is a common state of the vaginal microbiota characterized by increased bacterial diversity and decreased Lactobacillus abundance. Using untargeted metaproteomics, we identified host and bacterial proteins in a set of 29 cervicovaginal lavage samples from people with and without BV which revealed functions and interactions that likely contribute to BV.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervicovaginal Fluid

DISEASE(S): Bacterial Vaginosis

SUBMITTER: Elliot Lee  

LAB HEAD: David N

PROVIDER: PXD051980 | Pride | 2025-03-07

REPOSITORIES: Pride

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Publications

Optimizing metaproteomics database construction: lessons from a study of the vaginal microbiome.

Lee Elliot M EM   Srinivasan Sujatha S   Purvine Samuel O SO   Fiedler Tina L TL   Leiser Owen P OP   Proll Sean C SC   Minot Samuel S SS   Deatherage Kaiser Brooke L BL   Fredricks David N DN  

mSystems 20230623 4


Metaproteomics, a method for untargeted, high-throughput identification of proteins in complex samples, provides functional information about microbial communities and can tie functions to specific taxa. Metaproteomics often generates less data than other omics techniques, but analytical workflows can be improved to increase usable data in metaproteomic outputs. Identification of peptides in the metaproteomic analysis is performed by comparing mass spectra of sample peptides to a reference datab  ...[more]

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