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E cadherin appears to be an important on/off switch for initiating bile canaliculi formation

ABSTRACT: The mechanisms underlying cell polarization are fundamental in biology, yet they are not fully understood. This is particularly true for hepatocytes, which exhibit complex polarization distinct from other epithelial cells, enabling the formation of the bile canaliculi (BCs) network that is responsible for major liver function. To identify key proteins involved in hepatocytes polarization and BC formation, we performed a proteomic approach to compare the human hepatocyte cell line HepG2 to its sub clone HepG2/C3A which, shows much greater efficiency in forming mature BCs. We localized for the first time, LimA1 and Espin at the BC, which could play an important role at this place, and we confirmed the presence of NHE-RF1. Using a protein repression strategy directed against selected proteins, we highlighted the unique role of E cadherin in the initiation of BC formation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Hélène Diemer

LAB HEAD: Sarah Cianférani

PROVIDER: PXD052062 | Pride | 2026-02-09

REPOSITORIES: Pride

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			Action	DRS
	QX11107HD.raw	Raw
	QX11107HD.raw.mgf	Mgf
	QX11109HD.raw	Raw
	QX11109HD.raw.mgf	Mgf
	QX11111HD.raw	Raw

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