Dataset Information

Proteomic Analysis of Carotid Artery Plaques With and Without Vulnerable Features on Magnetic Resonance Angiography with Vessel Wall Imaging: A Pilot Study

ABSTRACT: Single-center, cohort study of plaques identified with and without vulnerable features on magnetic resonance angiography with vessel wall imaging. Proteomic analysis of these plaques demonstrates different protein composition. These proteins may be used for early detection and intervention or as pharmacologic targets in patients with higher risk for stroke carotid artery disease.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Carotid Artery

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Benjamin Madden

LAB HEAD: Young Erben

PROVIDER: PXD052120 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	Ex331_2021Aug29_Erben_P21024_1.mgf.gz	Mgf
	Ex331_2021Aug29_Erben_P21024_1.raw	Raw
	Ex331_2021Aug29_Erben_P21024_10.mgf.gz	Mgf
	Ex331_2021Aug29_Erben_P21024_10.raw	Raw
	Ex331_2021Aug29_Erben_P21024_11.mgf.gz	Mgf

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Proteomic analysis of carotid artery plaques with and without vulnerable features on magnetic resonance angiography with vessel wall imaging: a pilot study.

Madden Benjamin J BJ Polania-Sandoval Camilo C Pujari Ganesh P GP Mangalaparthi Kiran K KK Charlesworth M Cristine MC Prudencio Mercedes M Gendron Tania T Sandhu Sukhwinder J S SJS Nassar Aziza A Petrucelli Leonard L Meschia James F JF Pandey Akhilesh A Erben Young Y

JVS-vascular science 20250121

<h4>Objective</h4>Extracranial carotid artery pathology accounts for 15% to 20% of ischemic strokes. Advancements in magnetic resonance angiography (MRA) with vessel wall imaging (VWI) have enabled the identification of vulnerable plaques, aiding in risk stratification for neurovascular events. This pilot study aimed to identify proteins in plaques with and without vulnerable features on MRA with VWI.<h4>Methods</h4>Consecutive patients undergoing carotid endarterectomy were included in the stud ...[more]

PMID: 40034248

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Project description:BackgroundAccurate assessment of the vulnerability of carotid atherosclerotic plaques is crucial for stroke prevention. The three-dimensional (3D) magnetic resonance (MR) vessel wall imaging (VWI) has been increasingly employed to evaluate carotid plaques due to its extensive coverage and isotropic high spatial resolution. However, the accuracy of such techniques lacks validation by histology. Therefore, this study aims to validate the accuracy of 3D multi-contrast MR VWI with variable-flip-angle (VFA) and turbo spin echo (TSE) readout in identifying vulnerable carotid plaques, using histological analysis as a reference.MethodsTwenty-one male patients (mean age: 64.4 ± 7.2 years old) scheduled for carotid endarterectomy (CEA) were recruited in this study. All patients underwent carotid multi-contrast MR VWI, including 3D T1- and T2-weighted VFA-TSE sequences, as well as 3D time of flight (TOF) MR angiography (MRA), using a 3.0T MR system before surgery. Histological processing was performed for carotid plaque specimens. The presence or absence, along with the area measurements, of lipid-rich necrotic core (LRNC), intraplaque hemorrhage (IPH), and calcifications (CA) were independently evaluated on both MR images and histological sections. Cohen's kappa (κ) analysis was utilized to determine the agreement between 3D multi-contrast MR VWI and histology in identifying carotid plaque compositions before and after excluding compositions bellow certain size threshold. Spearman's correlation analysis was also conducted to assess the agreement in quantifying plaque compositions.ResultsA total of 81 slices of MR images were successfully matched with histological sections. Moderate to almost perfect agreements were observed between 3D MR VWI and histology in the identification of LRNC (κ: 0.85 and 0.89), IPH (κ: 0.65 and 0.69), and CA (κ: 0.46 and 0.62) before and after excluding compositions smaller than 0.79 mm2. Strong to very strong correlations were found in the quantification of plaque compositions including LRNC (r=0.88), IPH (r=0.80), and CA (r=0.74) between MR imaging and histology.ConclusionThe 3D VFA-TSE multi-contrast MR VWI is capable of accurately characterizing vulnerable carotid atherosclerotic plaques.