Dataset Information

Proteomics of E. coli extracellular vesicles

ABSTRACT: Escherichia coli A0 34/86 (EcO83) is a probiotic bacterium that is used in newborns to prevent nosocomial infections and diarrhoea. Mechanistically, EcO83 enhances innate immunity by inducing key inflammatory cytokines in vitro, and when administered intranasally, it modulates the recruitment of cells to the lung. This study investigates the immunomodulatory effects of EcO83-derived extracellular vesicles (EcO83-EVs) as a safer alternative to live bacteria. We isolated, purified, and characterised EcO83-EVs following the MISEV guidelines. The proteomics data of the EVs were compared with the proteomics data of cell extracts of the EcO83.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Johan Palmfeldt

LAB HEAD: Johan Palmfeldt

PROVIDER: PXD052178 | Pride | 2025-05-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Y2101-Brad-OMV-2-1.raw	Raw
	Y2103-Brad-OMV-2-2.raw	Raw
	Y2107--Brad-OMV-2-3.raw	Raw
	Y2109--Brad-OMV-2-5-12uL.raw	Raw
	Y2111--Brad-OMV-2-4-12uL.raw	Raw

Items per page:

1 - 5 of 11

Publications

Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells.

Razim Agnieszka A Zabłocka Agnieszka A Schmid Anna A Thaler Michael M Černý Viktor V Weinmayer Tamara T Whitehead Bradley B Martens Anke A Skalska Magdalena M Morandi Mattia M Schmidt Katy K Wysmołek Magdalena E ME Végvári Akos A Srutkova Dagmar D Schwarzer Martin M Neuninger Lukas L Nejsum Peter P Hrdý Jiri J Palmfeldt Johan J Brucale Marco M Valle Francesco F Górska Sabina S Wisgrill Lukas L Inic-Kanada Aleksandra A Wiedermann Ursula U Schabussova Irma I

Journal of extracellular vesicles 20241001 10

Escherichia coli A0 34/86 (EcO83) is a probiotic strain used in newborns to prevent nosocomial infections and diarrhoea. This bacterium stimulates both pro- and anti-inflammatory cytokine production and its intranasal administration reduces allergic airway inflammation in mice. Despite its benefits, there are concerns about the use of live probiotic bacteria due to potential systemic infections and gene transfer. Extracellular vesicles (EVs) derived from EcO83 (EcO83-EVs) might offer a safer alt ...[more]

PMID: 39429019

Dataset Information

Proteomics of E. coli extracellular vesicles

Dataset's files

Publications

Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Similar Datasets

Bacterial EVs as intranasal postbiotics: Detailed characterization and interaction with airway cells
2024-11-06 | PXD052553 | Pride

Gene expression data from bladder cells treated with Escherichia coli Extracellular Vesicles RNA
2020-07-08 | GSE148711 | GEO

Characteristics of two Saccharomyces cerevisiae strains and their extracellular vesicles as new candidates for probiotics.
2025-05-22 | PXD055073 | Pride

Comparative genomic hybridization of Saccharomyces boulardii and Saccharomyces cerevisiae to determine genomic differences
2006-11-10 | E-MAXD-15 | biostudies-arrayexpress

Does an immune response at the Ileum contribute to severity and development of helminth-diarrhoea in Merino sheep (ileum)?
2022-06-27 | GSE179149 | GEO

Exploring extracellular vesicles of probiotic yeast Saccharomyces boulardii CNCM I-745 as carriers of biologically active molecules transferred to human intestinal cells
2023-06-29 | PXD042660 | Pride