Proteomics

Dataset Information

0

DSSBU: An MS-Cleavable Analogue of the BS3 Cross-Linker


ABSTRACT: In this study, we introduce a new MS-cleavable cross-linker called disulfodisuccinimidyl dibutyric urea (DSSBU), which we developed in-house. DSSBU contains an N-hydroxysulfosuccinimide (sulfo-NHS) reactive group that primarily modifies lysine residues. It therefore serves as a water-soluble counterpart to the commercially available and widely used disuccinimidyl dibutyric urea (DSBU). To investigate the applicability of DSSBU, we compared the efficacy of four similar cross-linkers—bis[sulfosuccinimidyl] suberate (BS3), disuccinimidyl suberate (DSS), DSBU, and DSSBU—when used with the common model protein, bovine serum albumin (BSA).

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Petra Junková  

LAB HEAD: Josef Cvačka

PROVIDER: PXD052450 | Pride | 2025-12-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BS3_100_SET1_2-WATER_98-DMSO.mgf Mgf
BS3_100_SET1_2-WATER_98-DMSO.raw Raw
BS3_100_SET1_2-WATER_98-DMSO.zhrm Other
BS3_100_SET1_WATER.mgf Mgf
BS3_100_SET1_WATER.raw Raw
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