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TMT-Based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in the fine wool sheep foetus

ABSTRACT: Chinese Merino Sheep (Junken Type) is one of the fine wool sheep breeds in China, which is famous for producing fine wool. Wool quality including fibre diameter and length is the most concerned economic indicator for producers. Growth and development of secondary hair follicles (SFs) influences the wool quality. Understanding the formation mechanism of wool quality and related key proteins can help optimize and control the production of wool. However, The mechanism by which proteins mediate the development of SFs in the sheep foetus remains elusive. Here, based on the histomorphology of the SFs among four stages of foetus development (E75, E85, E95 and E105), the differential protein profiles of skin tissues were determined in three comparison pairs of foetus development (E75 versus E85, E85 versus E95, and E95 versus E105) by TMT-Based quantitative proteomics technology. With the help of proteomics technology, a total of 238, 35 and 348 differentially expressed proteins (DEPs) were identified in three comparison periods, respectively. In SFs development, focal adhesion, ECM−receptor interaction and estrogen signaling pathway, etc. played important role. COL1A1, THBS3, ITGA6, COL6A1 and THBS4 were potential candidate proteins in SF initiation. Overall, this study provided valuable proteomics data on the SFs development and a prospective understanding of the molecular mechanisms for wool quality traits in fine wool sheep.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ovis Aries

TISSUE(S): Skin

SUBMITTER: qiu lixia

LAB HEAD: Hua Yang

PROVIDER: PXD052470 | Pride | 2025-05-07

REPOSITORIES: Pride

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TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep.

Qiu Li-Xia LX Yu Qian Q Zhou Hua-Qian HQ Fan Wen-Hua WH Zheng Jing-Jing JJ Yang Yong-Lin YL Zhang Wen-Zhe WZ Cao Xin X Yang Hua H

PloS one 20250206 2

The development of secondary hair follicles influences the quality of sheep wool. However, the mechanism by which proteins mediate the fetal development of secondary hair follicles remains unknown. In this study, the histomorphology of secondary hair follicles was analyzed over four stages of fetal development (75, 85, 95, and 105 gestational days). TMT-based quantitative proteomics was used to compare the differential protein profiles of skin tissues between consecutive developmental periods (7 ...[more]

PMID: 39913466

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Project description:Sheep provide considerable materials for the animal fibre industry. Identifying genes of major effect for wool growth would offer strategies for improving the quality and increasing the yield of fine wool. In this study, we employed Agilent Sheep Gene Expression Microarray and proteomic technology to investigate the gene expression patterns of body side skin (more wool growing) in Aohan fine wool sheep (a Chinese indigenous breed) in comparison with groin skin (no wool growing) at the anagen stage of wool follicle. Microarray study revealed that 4772 probes were differentially expressed, including 2071 upregulated and 2701 downregulated probes in the comparisons of body side skin versus groin skin (S/G). The microarray results were verified by means of quantitative PCR. 1099 probes were assigned to unique genes/transcripts. The number of distinct genes/transcripts (annotated) was 926, of which 352 were up-regulated and 574 were down-regulated. In S/G, 13 genes were up-regulated by more than 10-fold, while 60 genes were down-regulated by more than 10-fold. Further analysis revealed that the majority of the genes possibly related to the wool growth could be assigned into the categories including regulation of cell division, intermediate filament, cytoskeletal part and growth factor activity. Several potential gene families might participate in hair growth regulation, including fibroblast growth factors, transforming growth factor-Î², WNTs, insulin-like growth factor, vascular endothelial growth factors and so on. Proteomic analysis also got 196 differentially expressed protein points, of which 121 were identified as single protein points. Furthermore, according to the results at both mRNA and protein levels, similar regulation mechanism of gene activity might be engaged during skin development and embryo development. One ram and two ewes of 12-month-old Aohan fine wool sheep were used in the microarray study. These animals were half sibs (sharing the same father). In August 2010, two areas of full-thickness skin were sampled from the same animal under local anaesthesia: body side skin (wool bearing) and groin skin (non-wool bearing) for microarray and proteomic experiments. The area of each sample was about 1 cm2. All samples were immediately put into collection tubes and stored in liquid nitrogen for RNA and protein extraction. A total of 15, 208 probes were spotted on this Agilent Sheep Gene Expression Microarray (Santa Clara, CA, USA).

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