Nucleotide metabolism-related host proteins RPB2 and UPP1 interacting with PEDV N proteins affect viral replication

ABSTRACT: Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic infectious disease targeting pig intestine that is widespread in the world, causing huge economic losses to pig industry. PEDV N protein is the core protein of PEDV, which locates in the cytoplasm and wraps the RNA genome of the virus to form spiral ribonucleoprotein (RNP). However, host proteins interacting with N proteins and their role in PEDV replication have not been fully elucidated. In this study, PEDV-N labeled antibodies were immunoprecipitated (Co-IP) and combined with LC-MS/MS to screen host proteins interacting with N proteins.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Kidney Cell, Kidney

DISEASE(S): Diarrhea

SUBMITTER: Qiyuan Kuang  

LAB HEAD: Guihong Zhang

PROVIDER: PXD052564 | Pride | 2024-06-07

REPOSITORIES: Pride