Dataset Information

Campylobacter rsmA mutantIncreased motility in Campylobacter jejuni and changes in its virulence, fitness and morphology following protein expression on ribosomes with altered RsmA methylation

ABSTRACT: Infection with Campylobacter jejuni is the major cause of human gastroenteritis in the United States and Europe, leading in many cases to debilitating autoimmune sequelae. While considerable progress has been made in detailing the infectious cycle of C. jejuni, a full understanding of the molecular mechanisms responsible for virulence remains to be elucidated. Here, we apply a novel approach by modulating protein expression on the pathogen’s ribosomes by inactivating a highly conserved rRNA methyltransferase. Loss of the RsmA methyltransferase results in a more motile strain with greater adhesive and cell invasive properties. These phenotypical effects correlate with enhanced expression of specific proteins related to flagellar formation and function, together with enzymes involved in cell wall/membrane and amino acid synthesis. Despite the enhancement of certain virulent traits, the null-strain grows poorly on minimal media and is rapidly out-competed by the wild-type strain. Complementation with an active copy of the rsmA gene rescues most of the traits changed in the mutant. However, the complemented strain overexpresses rsmA and displays new flaws including loss of the spiral cell shape that is distinctive for C. jejuni. Proteins linked with altered virulence and morphology are identified here by mass spectrometry proteomic analyses of the strains.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni 81-176 Bacteria

DISEASE(S): Campylobacteriosis

SUBMITTER: Agnieszka Guz

LAB HEAD: Agnieszka Sałamaszyńska-Guz

PROVIDER: PXD052567 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	MRL_ASG_E_20220927_Exp2_F.msf	Msf
	MRL_ASG_E_20220927_Exp2_F1.raw	Raw
	MRL_ASG_E_20220927_Exp2_F10.raw	Raw
	MRL_ASG_E_20220927_Exp2_F11.raw	Raw
	MRL_ASG_E_20220927_Exp2_F12.raw	Raw

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Publications

Increased Motility in Campylobacter jejuni and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation.

Sałamaszyńska-Guz Agnieszka A Murawska Małgorzata M Bącal Paweł P Ostrowska Agnieszka A Kwiecień Ewelina E Stefańska Ilona I Douthwaite Stephen S

International journal of molecular sciences 20240910 18

Infection with Campylobacter jejuni is the major cause of human gastroenteritis in the United States and Europe, leading to debilitating autoimmune sequelae in many cases. While considerable progress has been made in detailing the infectious cycle of C. jejuni, a full understanding of the molecular mechanisms responsible for virulence remains to be elucidated. Here, we apply a novel approach by modulating protein expression on the pathogen's ribosomes by inactivating a highly conse ...[more]

PMID: 39337285

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Project description:Background: The food-borne pathogen Campylobacter is one of the most important zoonotic pathogens. Compared to other zoonotic bacteria, Campylobacter species are quite susceptible to environmental or technological stressors. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress response in Campylobacter (C.) jejuni are already known, information about the heat stress response in the related species C. coli and C. lari are still unknown. Results: The stress response to elevated temperatures (46°C) was investigated by survival assays and whole transcriptome analyses for the strain C. jejuni NCTC11168, C. coli RM2228 and C. lari RM2100. While C. jejuni showed highest thermotolerance followed by C. lari and C. coli, none of the strains survived at this temperature for more than 24 hours. Transcriptomic analyses revealed that only 3 % of the genes in C. jejuni and approx. 20 % of the genes of C. coli and C. lari were differentially expressed after heat stress, respectively. The transcriptomic profiles showed enhanced gene expression of several chaperones like dnaK, groES, groEL and clpB in all strains, but differences in the gene expression of transcriptional regulators like hspR, perR as well as for genes involved in metabolic pathways, translation processes and membrane components. However, the function of many of the differentially expressed gene is unknown so far. Conclusion: We could demonstrate differences in the ability to survive at elevated temperatures for C. jejuni, C. coli and C. lari and showed for the first time transcriptomic analyses of the heat stress response of C. coli and C. lari. Our data suggest that the heat stress response of C. coli and C. lari are more similar to each other compared to C. jejuni, even though on genetic level a higher homology exists between C. jejuni and C. coli. This indicates that stress response mechanisms described for C. jejuni might be unique for this species and not necessarily transferable to other Campylobacter species.

Dataset Information

Campylobacter rsmA mutantIncreased motility in Campylobacter jejuni and changes in its virulence, fitness and morphology following protein expression on ribosomes with altered RsmA methylation

Dataset's files

Publications

Increased Motility in <i>Campylobacter jejuni</i> and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation.

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