Human OTX2 TurboID Analysis in Transfected HDMB03 Cells

ABSTRACT: HDMB03 cells were transiently transfected with plasmids expressing NLS-TurboID or 3xHA-TurboID-OTX2 under the control of a strong CAG promoter. After a 15-minute pulse with 500 µM exogenous biotin, RIPA lysates were prepared and processed with streptavin-sepharose to capture biotinylated proteins that were identified by using mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Brain Cancer

SUBMITTER: Brad Doble  

LAB HEAD: Tamra Erin Ogilvie

PROVIDER: PXD052687 | Pride | 2024-05-31

REPOSITORIES: Pride