Cysteine capping with CAM reagent

ABSTRACT: CAM reagent was synthesized and used to uniquely convert cysteine residues, under mild conditions, into trypsin-recognsable sites. We apply this cysteine-capping methodology in the identification of missense variants in HCT-15 cell lines.2-bromoethylamine (BEA)was used as controls, for cysteine capping and subjected to similar trypsin treatment.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Samuel Ofori  

LAB HEAD: Dr Keriann Marie Backus

PROVIDER: PXD052935 | Pride | 2025-03-13

REPOSITORIES: Pride