Dataset Information

Carotid endarterectomy tissue and tissue-derived extracellular vesicle proteomes from symptomatic and asymptomatic patients

ABSTRACT: This study sought to compare how the tissue extracellular vesicle proteome of carotid atherosclerotic plaques differs between symptomatic and asymptomatic patients. Carotid arteries samples were collected from symptomatic and asymptomatic patients undergoing endarterectomies. The specimen was grossly dissected in to 1) the maximally diseased plaque zone and 2) adjacent marginal zones. Extracellular vesicles were isolated from these matched tissue regions from each specimen using collagenase digestion, size exclusion chromatography, and density gradient ultracentrifugation. Label-free proteomics was performed on both the intact tissues (N=5) and the tissue derived EVs (N=23: symptomatic patients N=13, asymptomatic patients N=10).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Carotid Atherosclerotic Plaque, Carotid Artery Segment

DISEASE(S): Atherosclerosis

SUBMITTER: Mandy Turner

LAB HEAD: Kathryn L

PROVIDER: PXD052938 | Pride | 2025-06-30

REPOSITORIES: Pride

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Publications

Multiomic Landscape of Extracellular Vesicles in Human Carotid Atherosclerotic Plaque Reveals Endothelial Communication Networks.

Raju Sneha S Turner Mandy E ME Cao Christian C Abdul-Samad Majed M Punwasi Neil N Blaser Mark C MC Cahalane Rachel M E RME Botts Steven R SR Prajapati Kamalben K Patel Sarvatit S Wu Ruilin R Gustafson Dakota D Galant Natalie J NJ Fiddes Lindsey L Chemaly Melody M Hedin Ulf U Matic Ljubica L Seidman Michael A MA Subasri Vallijah V Singh Sasha A SA Aikawa Elena E Fish Jason E JE Howe Kathryn L KL

Arteriosclerosis, thrombosis, and vascular biology 20250529 7

<h4>Background</h4>Carotid atherosclerosis is orchestrated by cell-cell communication that drives progression along a clinical continuum (asymptomatic to symptomatic). Extracellular vesicles (EVs) are cell-derived nanoparticles representing a new paradigm in cellular communication. Little is known about their biological cargo, cellular origin/destination, and functional roles in human atherosclerotic plaque.<h4>Methods</h4>EVs were enriched via size exclusion chromatography from human carotid en ...[more]

PMID: 40438929

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