Tear Proteomics Analysis in Children and Adolescents with Type 1 Diabetes

ABSTRACT: Fifty-six children and adolescents with type 1 diabetes at least one year after diagnosis, aged 6-17 years old and fifty-six healthy age- and sex-matched subjects were enrolled in this cross-sectional study. Tear samples were collected using Schirmer strips placed on the lower eyelid. The proteomic analysis was based on a detergent-assisted protein extraction and their digestion from the tears, analysis of the tryptic peptides with LC-MS/ enabling the identification, and quantification of the Shirmer strip protein content via DIA-NN, and subsequently the statistical and bioinformatic analysis using the R and Metascape enrichment analysis tool.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tear

DISEASE(S): Type 1 Diabetes Mellitus

SUBMITTER: Martina Samiotaki  

LAB HEAD: Nicolas C. Nicolaides

PROVIDER: PXD052994 | Pride | 2024-10-17

REPOSITORIES: Pride