Dataset Information

Time-based quantitative proteomic and phosphoproteomic analysis of A549-ACE2 cells during SARS-CoV-2 infection

ABSTRACT: The outbreak of COVID-19, a disease caused by severe acute respiratory syndrome coronavirus 2, led to an ongoing pandemic with devastating consequences for the global economy and human health. With the globalspread of SARS-CoV-2, multidisciplinary initiatives were launched to explore new diagnostic, therapeutic, and vaccination strategies. From this perspective, proteomics could help to understand the mechanisms associated with SARS-CoV-2 infection and to identify new therapeutic targets for antiviral drug repurposing and/or discovery. A TMT-based quantitative proteomics and phosphoproteomics analysis was performed to study the proteome remodeling of human lung alveolar cells transduced to express human ACE2 (A549-ACE2) after infection with SARS-CoV-2. Targeted PRM analysis was performed to assess the detectability in serum and prognostic value of selected proteins. A total of 6802 proteins and 6428 phospho-sites were identified in A549-ACE2 cells after infection with SARS-CoV-2. Regarding the viral proteome, 8 proteins were differentially expressed after 6 h of infection and reached a steady state after 9 h. In addition, we detected several phosphorylation sites of SARS-CoV-2 proteins, including two novel phosphorylation events at S410 and S416 of the viral nucleoprotein.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Sergio Ciordia

LAB HEAD: Fernando Corrales

PROVIDER: PXD053240 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20211005_12_SARS_Cells_TMT16plex_SDB-I_Fr3.raw	Raw
	20211005_15_SARS_Cells_TMT16plex_SDB-I_Fr4.raw	Raw
	20211005_18_SARS_Cells_TMT16plex_SDB-I_Fr5.raw	Raw
	20211005_21_SARS_Cells_TMT16plex_SDB-I_Fr6.raw	Raw
	20211005_24_SARS_Cells_TMT16plex_SDB-I_Fr7.raw	Raw

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Publications

Dynamic Cellular Proteome Remodeling during SARS-CoV-2 Infection. Identification of Plasma Protein Readouts.

Dos Santos Fátima Milhano FM Vindel-Alfageme Jorge J Ciordia Sergio S Castro Victoria V Orera Irene I Garaigorta Urtzi U Gastaminza Pablo P Corrales Fernando F

Journal of proteome research 20241126 1

The outbreak of COVID-19, led to an ongoing pandemic with devastating consequences for the global economy and human health. With the global spread of SARS-CoV-2, multidisciplinary initiatives were launched to explore new diagnostic, therapeutic, and vaccination strategies. From this perspective, proteomics could help to understand the mechanisms associated with SARS-CoV-2 infection and to identify new therapeutic options. A TMT-based quantitative proteomics and phosphoproteomics analysis was per ...[more]

PMID: 39593238

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