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Characterisation of decellularized extracellular matrix hydrogels

ABSTRACT: This study investigated the development and characterization of decellularized extracellular matrix (dECM) hydrogels tailored for the bio-fabrication of female reproductive tissues, specifically targeting cortex, endometrium, medulla, and oviduct tissues. We aimed to evaluate the cytocompatibility, biomechanical properties, and overall efficacy of these dECMs in promoting cell viability, proliferation, and differentiation.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Jan Bernd Stöckl

LAB HEAD: Thomas Fröhlich

PROVIDER: PXD053248 | Pride | 2026-03-12

REPOSITORIES: Pride

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			Action	DRS
	CF_DIA_1ug.raw	Raw
	CFp2_DIA_1ug.raw	Raw
	CFp3_DIA_1ug.raw	Raw
	CFp4_DIA_1ug.raw	Raw
	CFp5_DIA_1ug.raw	Raw

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Publications

Developing and characterising bovine decellularized extracellular matrix hydrogels to biofabricate female reproductive tissues.

Ribes Martinez E E Franko Y Y Franko R R Ferronato G A GA Viana A E S AES Windenbach E E Stoeckl J B JB Fröhlich T T Ferraz M A M M MAMM

Acta biomaterialia 20250307

This study investigated the development and characterization of decellularized extracellular matrix (dECM) hydrogels tailored for the biofabrication of female reproductive tissues, specifically targeting ovarian cortex, endometrium, ovarian medulla, and oviduct tissues. We aimed to evaluate the cytocompatibility, biomechanical properties, and overall efficacy of these dECMs in promoting cell viability, proliferation, and morphology using the bovine model. Bovine species provide a valuable model ...[more]

PMID: 40058619

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LC-MS/MS of Versatile Decellularized Human Lung Extracellular Matrix-Derived Hydrogels

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Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning with decellularized matrix

Project description:As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as fibroblast-like shape. dECM expanded SDSCs yielded large pellets with intensive staining of type II collagen and sulfated GAGs, which was supported by both biochemical data and real-time PCR results. Our results also hint at lower levels of inflammatory genes and how they might be responsible for enhanced chondrogenic differentiation in dECM expanded SDSCs. Despite an increase of type X collagen in chondrogenically induced cells, dECM expanded cells had significantly lower potential for endochondral bone formation. Both Wnt and MAPK signals were actively involved in both expansion and chondrogenic induction of dECM expanded cells. dECM expanded human SDSCs could be a potential cell source for autologous cartilage repair Adult human synovial fibroblasts (4 donors, two male and two female, average 43 years old, all had no known joint disease) were grown for one passage on plastic flasks (P cells) or plastic flasks pre-coated with decellularized extracellular matrix (E cells). Aliquots were centrifuged and pellets cultured for 35 days in serum-free chondrogenic medium (P pellet or E pellet). There are no replicates.

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Bioactive decellularized extracellular matrix-based hydrogel supports human adipose tissue-derived stem cell maintenance and fibrocartilage phenotype

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Evolutionarily conserved sequence motif analysis guided development of chemically defined hydrogels for therapeutic angiogenesis

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Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks

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Comparative proteomic analysis of the composition of decellularized extracellular matrix (dECM) and dECM-based inks as compared to the native tissue

Project description:Regenerative medicine and tissue engineering approaches based on the use of 3D-bioprinted decellularized extracellular matrix (dECM) present the advantage of a relatively biomolecule-rich matrix, which directs cell growth and differentiation in a tissue-specific manner. However, little is known about the composition changes that occur with standard processing of dECM-based inks. To characterize this process, six porcine tissues/tissue layers (artery, breast, dermis, epidermis, muscle and nerve) were independently decellularized via chemical, mechanical and enzymatic processes and the resulting dECMs formulated into biocompatible inks, to serve as source biomaterials for 3D printing. A comparative liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomic analysis was carried out for native tissue, decellularized and formulated ECMs, and the resulting complexity of the matrisome analyzed. A core matrisome was found to overlap in all decellularized tissues, as well as tissue-specific components that correlated with predicted functional (gene ontology-based) definitions. The proportion of collagens (mostly the α1 chains of collagen type I and III) increased in the final processing step (inks) as compared to the native ECM and dECM stages. Overall, a median of 55 matrisomal proteins (range 45-126) was detected in the dECM-derived inks. This complexity is far superior in terms of mimicking the composition of native tissue to non-dECM-based inks. Our results support the use of dECM-based inks and biomaterials in mimicking native tissue ECM complexity.

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A Comparative Proteomic Study of Decellularized Extracellular Matrix Biomaterials Derived from Porcine Vocal Fold and Small Intestinal Submucosa Tissue Source

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2023-02-01 | PXD033621 | Pride

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