Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum

ABSTRACT: The present work developed a non-affinity-based chromatographic method to enrich MUC16 from serum. The enriched MUC16 sample was further processed using a Midi Top 14 abundant protein depletion column. Peptides identified using bottom-up proteomics yielded 1–8% coverage of MUC16. Additionally, MUC16 was detected in samples containing less than the clinical cut-off level of CA125 (35 U/mL), suggesting that this strategy of enrichment and bottom-up proteomics can enable analysis of CA125 from serum of individuals with early-stage ovarian cancer and those whose tumors express CA125 (MUC16) at low levels.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Rebecca Whelan  

LAB HEAD: Rebecca Whelan

PROVIDER: PXD053289 | Pride | 2024-10-17

REPOSITORIES: Pride