Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in nutrient-replete cells to post-transcriptionally repress starvation-induced pathways

ABSTRACT: Degradation of many yeast mRNAs involves decapping by Dcp1:Dcp2. Previous studies on decapping activators Edc3 and Scd6 suggested limited roles in promoting mRNA decay in yeast cells. RNA-seq analysis of mutants lacking one or both proteins reveals that Scd6 and Edc3 have largely redundant functions in targeting numerous mRNAs for degradation, whose roles are masked in the single mutants. These mRNAs are also frequently targeted by decapping activators Dhh1 and Pat1 and the decapping enzyme itself, and the data support the proposal that Scd6/Edc3 act interchangeably to recruit Dhh1 to Dcp2 independently of Pat1. Ribosome profiling shows that functional redundancy of Scd6 and Edc3 and cooperation with Dhh1/Pat1 extends to the translational repression of particular transcripts, including a cohort of poorly translated mRNAs displaying interdependent regulation by all four factors. Scd6/Edc3 also partner with Dhh1/Pat1 in nutrient-replete cells for post-transcriptional repression of proteins required for respiration and catabolism of non-optimal carbon sources, which are normally expressed only in cells deprived of glucose. As such, simultaneously eliminating Scd6/Edc3 increases mitochondrial membrane potential and elevates metabolites of the tricarboxylic acid and glyoxylate cycles critical for growth in cells growing without glucose. Thus, Scd6/Edc3 act redundantly and together with decapping activators Dhh1 and Pat1 to post-transcriptionally adjust gene expression to nutrient availability.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Rakesh Kumar  

LAB HEAD: Alan G. Hinnebusch

PROVIDER: PXD053307 | Pride | 2025-10-30

REPOSITORIES: Pride