Identification of proteins in SDS-PAGE gel by LC-MSMS

ABSTRACT: Recombinant antibodies were purified from culture supernatants derived from the Dictyostelium discoideum model and loaded into an SDS-PAGE electrophoresis gel. The electrophoretic profile of the anti-alpha tubulin, anti-CISD1, and anti-CD8β antibodies reveals a band at 35 kDa, likely corresponding to a degradation product of the recombinant antibody. Additionally, a non-specific band appears under all conditions at 170 kDa. The objective of this analysis was to characterize these bands by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Cyril Guilhen  

LAB HEAD: Cyril Guilhen

PROVIDER: PXD053343 | Pride | 2025-04-11

REPOSITORIES: Pride