Dataset Information

Identification of Scedosporium boydii specific antigens with potential diagnostic interest in patients with cystic fibrosis

ABSTRACT: Fungal infections pose a significant threat to patients with cystic fibrosis (pwCF). Particularly those caused by Scedosporium/Lomentospora species, being the second most common filamentous fungi in the respiratory tract of these patients after Aspergillus. The current serodiagnostic methods for detecting Scedosporium/Lomentospora, such as Counterimmunoelectrophoresis and ELISA using crude extracts, are neither standardized nor commercially available, and they suffer from cross-reactivity with other fungal species. This study aimed to identify specific antigens of Scedosporium boydii with potential utility for serodiagnosis in pwCF. The antigenic profile of S. boydii was analyzed by combining Two-Dimensional Polyacrylamide Gel Electrophoresis and immunoblotting with sera from pwCF with microbiological cultures of respiratory samples positive for Scedosporium/Lomentospora (Scedo+), and sera from mice intravenously infected with S. boydii. The fungal antigens specifically recognized by pwCF Scedo+ were: 6 phosphogluconate dehydrogenase (Pgd), 40S ribosomal protein S1 (Rps1) and S18 (Rps18), Small glutamine-rich tetratricopeptide repeat-containing protein (Sgta), Cytochrome c peroxidase (Ccp), Mannitol-1-phosphate 5-dehydrogenase (Mpd), L xylulose reductase (Lxr), Tetrahydroxynaphthalene reductase (Thnr), Heat shock 70 kDa protein (Hsp70), Proteasome subunits alpha (Psma6) and beta (Psmb2), Peroxiredoxin-1 (Prdx1), ATP dependent Clp protease proteolytic subunit (Clpp), 3 ketoacyl-CoA thiolase (Kat), 4 aminobutyrate aminotransferase (Abat), Succinyl CoA:3-ketoacid CoA transferase 1 (Scot) and Phosphoenolpyruvate carboxykinase (Pck). The fungal antigens specifically recognized by infected mice were: Hsp70, S5 DRBM domain-containing ribosomal protein (Rps5), mitochondrial NFU1 iron-sulfur cluster scaffold-like protein (Nfu1), 60S ribosomal protein L8 (Rpsl8), phosphomannomutase (Pmm) and proteasome subunit alpha type 5 protein (Psma5).

INSTRUMENT(S):

ORGANISM(S): Fungi Scedosporium Boydii

SUBMITTER: Aitziber Antoran

LAB HEAD: Andoni Ramirez

PROVIDER: PXD053392 | Pride | 2025-09-29

REPOSITORIES: Pride

ACCESS DATA

Publications

Immunoproteomic Identification of Scedosporium boydii Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.

Martin-Souto Leire L Abio-Dorronsoro Lucia L Areitio Maialen M Aparicio-Fernandez Leire L Rodriguez-Erenaga Oier O Martin-Gomez Maria Teresa MT Antoran Aitziber A Rementeria Aitor A Buldain Idoia I Ramirez-Garcia Andoni A

Journal of proteome research 20250925

Fungal infections caused by Scedosporium/Lomentospora species are a significant threat to patients with cystic fibrosis (pwCF), ranking as the second most common filamentous fungi in their airways after Aspergillus. Current serodiagnostic methods, such as counterimmunoelectrophoresis and ELISA using crude extracts, lack standardization and commercial availability and often show cross-reactivity with other fungi. This study aimed to identify specific Scedosporium boydii</i ...[more]

PMID: 40997942

Similar Datasets

Project description:Of the species of Trichinella spp. that may affect human health, Trichinella britovi is the second most common. As an early diagnosis of trichinellosis is crucial for effective treatment, it is important to identify sensitive, specific and common antigens of adult T. britovi worms and muscle larvae. The present study was undertaken to uncover the stage-specific and common proteins of T. britovi that hold promise for specific diagnostics. To that end, the somatic extracts obtained from two developmental stages, muscle larvae (ML) and adult worms (Ad), were separated using two-dimensional gel electrophoresis (2DE) coupled with immunoblot analysis. The positively-visualized protein spots specific for each stage were identified through liquid chromatography-tandem mass spectrometry (LC-LC/MS). A total of 272 spots were detected in the proteome of T. britovi adult worms (Ad) and 261 in the muscle larvae (ML). The somatic extracts from Ad and ML were specifically recognized by pig T. britovi-infected swine sera at 10 days post infection (dpi) and 60 dpi, with a total of 70 prominent protein spots. According to immunoblotting patterns and LC-MS/MS results, the immunogenic spots recognized by different pig T. britovi-infected sera were divided into three groups for the two developmental stages: adult stage-specific proteins, muscle larvae stage-specific proteins, and common proteins. Forty-five Ad proteins (29 Ad-specific and 16 common) and thirteen ML proteins (nine ML-specific and four common) cross-reacted with sera at 10 dpi. Many of the proteins identified in Ad (myosin-4, myosin light chain kinase, paramyosin, intermediate filament protein B, actin-depolymerizing factor 1, and calreticulin) are involved in structural and motor activity. Among the most abundant proteins identified in ML were 14-3-3 protein zeta, actin-5C, ATP synthase subunit d, deoxyribonuclease-2-alpha, poly-cysteine and histide-tailed protein, enolase, V-type proton ATPase catalytic and serine protease 30. Heat shock protein, intermediate filament protein ifa-1 and intermediate filament protein B were identified in both proteomes. The current study represents the first immunoproteomic identification of the antigenic proteins of adult worm and muscle larvae of T. britovi. These results provide a valuable basis for the development of diagnostic methods. The identification of common components among two developmental stages of T. britovi may be useful in the preparation of parasitic antigens in recombinant forms for diagnostic use.

			Action	DRS
	H1.msf	Msf
	H1.raw	Raw
	H10.msf	Msf
	H10.raw	Raw
	H11.msf	Msf

Dataset Information

Identification of Scedosporium boydii specific antigens with potential diagnostic interest in patients with cystic fibrosis

Dataset's files

Publications

Immunoproteomic Identification of <i>Scedosporium boydii</i> Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Identification of Scedosporium boydii specific antigens with potential diagnostic interest in patients with cystic fibrosis

Dataset's files

Publications

Immunoproteomic Identification of &lt;i&gt;Scedosporium boydii&lt;/i&gt; Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Immunoproteomic Identification of <i>Scedosporium boydii</i> Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.