Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human HepG2 cell isonicotinylation proteomic

ABSTRACT: Qualitative proteomic research by isonicoacylation enrichment technique and high resolution liquid chromatography-mass spectrometry.In this project, a qualitative study of isonicotinylation proteomics was carried out. A total of 35 were identified in this study 44 isonicotinylation sites on individual proteins. We conducted a systematic bioinformatics analysis of the modified proteins,It includes Motif analysis, annotation of modified proteins, functional classification and functional enrichment analysis

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: li yixiao

LAB HEAD: Yixiao Li

PROVIDER: PXD053507 | Pride | 2024-08-08

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	XB03952PI_isonico_Fr1.raw	Raw
	XB03952PI_isonico_Fr2.raw	Raw
	XB03952PI_isonico_Fr3.raw	Raw
	XB03952PI_isonico_Fr4.raw	Raw
	checksum.txt	Txt

Items per page:

1 - 5 of 6

Similar Datasets

Human HeLa cell isonicotinylation proteomic

Project description:Qualitative proteomic research by isonicoacylation enrichment technique and high resolution liquid chromatography-mass spectrometry.In this project, a qualitative study of isonicotinylation proteomics was carried out. A total of 2792 were identified in this study 11,442 isonicotinylation sites on individual proteins. We conducted a systematic bioinformatics analysis of the modified proteins,It includes Motif analysis, annotation of modified proteins, functional classification and functional enrichment analysis

2024-08-08 | PXD050143 | Pride

HepG2 secretome tagged with the sugar analogs GalNAz and GalNAzMe

Project description:Chemically modified analogs of GalNAc, termed GalNAz or GalNAzMe, were incorporated in secreted glycoproteins. The compounds contain bioorthogonal, clickable functional groups that allowed for enrichment using biotin. Chemical glycoproteomics was performed after on-bead digestion and cleavage of glycopeptides from the solid support.

2020-09-14 | PXD020648 | Pride

Quercetin effect on gene expression in HepG2 hepatocellular cancer cells

Project description:Quercetin is a flavonol modifying numerous cell processes with potent antiproliferative effects on cancer cell-lines. The aim of this study was to explore by gene-array analysis the effect of quercetin on cancer-related gene expression in HepG2 cells, followed by verification with RT-PCR and analysis of the expected phenotypic changes (migration, cell cycle, cell proliferation). Quercetin induces significant changes on cell-adhesion related genes, leading to reduced migratory capacity and disorganization of the actin cytoskeleton. Several genes related to DNA functions, cellular metabolism and signal-transducer activities were also modified, while an early effect on G–protein related cascades possibly via protease-activated receptor 2 and phospholipase C-?1 was identified. Cyclin-D associated events in G1 and ubiquitin-dependent degradation of cyclin-D1 were also affected, resulting in cell-cycle arrest without activation of apoptosis pathways. In conclusion quercetin (3?M) exerts its cellular effects by modifying numerous genes related to mechanisms involved in cancer initiation and promotion. HepG2 cells were serum starved for 24 hours and were then treated with serum free medium with or without quercetin (3?M). Total RNA was collected at 2, 4, 12 and 24 hours and was used for gene-array experiments.

2009-03-11 | E-GEOD-15162 | biostudies-arrayexpress

Expression analysis of HepG2, HepG2-slug and HepG2-slug on Matrigel

Project description:Investigation of whole genome gene expression level changes in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Whole genome gene expression level changes have been compared in hepatocellular carcinoma cell line hepG2 in regular culture, hepG2-slug in regular culture and hepG2-slug on Matrigel. Roche NimbleGen micro-array analysis was employed to assess global genome expression in HepG2 in regular culture, HepG2-slug in regular culture and HepG2-slug on Matrigel. The results demonstrated that the up-regulated genes and the down-regulated genes increased significantly when HepG2-slug cells with VM forming ablity were cultured on Matrigel and formed VM.

2012-09-21 | E-GEOD-41026 | biostudies-arrayexpress

differentially expressed N-glycopeptides in HepG2 related to LO2 cell line

Project description:With isotopically dimethyl labeling, ZIC-HILIC enrichment, C18 pre-fractionation, differentially expressed N-glycosylation in HCC HepG2 cells (relative to normal liver LO2 cells) were analyzed using penta-HILIC-MS/MS.

2019-05-31 | PXD012424 | Pride

Erastin and ferrostatin induced protein expression change in HepG2 cells

Project description:Project description: HepG2 cells were treated with DMSO, erastin and ferrostatin, respectively. Protein was extracted and separated using SDS-PAGE electrophoresis. MS analysis was performed and protein was identified and quantified using Proteome Discoverer™ 1.3 software using the SEQUEST® search engine.

2019-11-12 | PXD010761 | Pride

Identification of ubiquitinated proteins in lung adenocarcinoma and normal lung tissues

Project description:To identify ubiquitinated modified proteins of lung adenocarcinoma, we collected five pairs of lung adenocarcinoma and normal lung tissues from the clinic for analysis

2022-01-12 | PXD027001 | Pride

Acetylome analysis of HepG2 cells after treatment with PBS or LukS-PV

Project description:We previously reported that LukS-PV, a component of Panton-Valentine interleukin (PVL), has anti-leukaemic activity, inducing differentiation, increasing apoptosis and inhibiting proliferation of acute myeloid leukaemia (AML) cells. In addition, our study showed that LukS-PV could inhibit the progression of hepatocellular carcinoma (HCC) by targeting C5aR. To investigate the biological mechanisms involved, we performed acetylome analysis on PBS/LukS-PV-treated HCC cells to search for important downstream regulators.

2026-01-02 | PXD057287 | Pride

HBX-HepG2 versus Control-HepG2

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells under UV stress The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments. This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-16 | E-GEOD-15554 | biostudies-arrayexpress

HBX-HepG2 versus Control-HepG2 (no UV)

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells. Free of UV sensitization. The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments (no UV). This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-24 | E-GEOD-17474 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data