Dataset Information

Proteomic response of macrophages to human cancer Raji cells

ABSTRACT: In order to determine changes in phosphorylation events in macrophages upon phagocytosis of cancer cells, mouse macrophages were co-cultured with human Raji cancer cells. Macrophages were collected as 0, 1, 15, 60 and 120 minutes after incubation. Phosphosite abundances were compared between timepoints to determine differential phospho signaling over time.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Krystine Mansfield

LAB HEAD: Patrick Pirrotte

PROVIDER: PXD053542 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	093_0001_01A_R_MultiTMTcorrect_30JUL20_CELL_MOU_LU2_0000_PA_300MIN_AM_RM_01.msf	Msf
	093_0001_01A_R_MultiTMTcorrect_30JUL20_CELL_MOU_LU2_0000_PA_300MIN_AM_RM_01.pdResult	Other
	rm200730ol006.raw	Raw
	rm200730ol007.raw	Raw

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Publications

Phosphoproteomic Profiling Reveals mTOR Signaling in Sustaining Macrophage Phagocytosis of Cancer Cells.

Wang Bixin B Cao Xu X Garcia-Mansfield Krystine K Zhou Jingkai J Manousopoulou Antigoni A Pirrotte Patrick P Wang Yingyu Y Wang Leo D LD Feng Mingye M

Cancers 20241219 24

<b>Background</b>: Macrophage-mediated cancer cell phagocytosis has demonstrated considerable therapeutic potential. While the initiation of phagocytosis, facilitated by interactions between cancer cell surface signals and macrophage receptors, has been characterized, the mechanisms underlying its sustentation and attenuation post-initiation remain poorly understood. <b>Methods</b>: Through comprehensive phosphoproteomic profiling, we interrogated the temporal evolution of the phosphorylation pr ...[more]

PMID: 39766137

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