Proteomics

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TurboID-mediated proximity labeling of DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans

ABSTRACT: TurboID-mediated proximity labeling of the aging and stress regulatory transcription factor DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans. Endogenous DAF-16 was C-terminally tagged with the biotin ligase TurboID using CRISPR/Cas9- genome editing. In vivo proximity labeling of the DAF-16 interactome was analyzed at normal and low insulin/IGF-1 signaling conditions. The biotin-labeled DAF-16 interactome was affinity purified using streptavidin resin and analyzed with mass spec.

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Markus Hartl  

LAB HEAD: Mario de Bono

PROVIDER: PXD053591 | Pride | 2025-12-29

REPOSITORIES: Pride

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20220316_E2_RSLC2_Artan_deBono_MPLGmbH_Academic_D16D2_40p.raw Raw
20220316_E2_RSLC2_Artan_deBono_MPLGmbH_Academic_D16_17c5p.raw Raw
20220316_E2_RSLC2_Artan_deBono_MPLGmbH_Academic_M_45p.raw Raw
20220413_E2_RSLC2_Artan_deBono_MPLGmbH_Academic_D16D2_S2_16c5p.raw Raw
20220413_E2_RSLC2_Artan_deBono_MPLGmbH_Academic_D16_S2_20p.raw Raw
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Publications

Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins.

Artan Murat M   Schoen Hanna H   de Bono Mario M  

Nature communications 20251211 1

Insulin/insulin-like growth factor signaling inhibits FOXO transcription factors to control development, homeostasis, and aging. Here, we use proximity labeling to identify proteins interacting with the C. elegans FOXO DAF-16. We show that in well-fed, unstressed animals harboring active insulin signaling, DAF-16 forms a complex with the PAR-1/MARK serine/threonine kinase, a key regulator of cell polarity. PAR-1 inhibits DAF-16 accumulation and promotes DAF-16 phosphorylation at S249, at a conse  ...[more]

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