Proteomics

Dataset Information

Absolute quantification analysis of ubiquitin chains attached to Nrf1 purified from NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2.

ABSTRACT: We examined ubiquitin-linkages on Nrf1 purified from cell lysates of NGLY1-KO cells overexpressing Nrf1-FLAG with HA-FBS2. The ubiquitin linkage types were quantified using the LC-MS/MS-based absolute quantification method, ubiquitin parallel reaction monitoring (Ub-AQUA/PRM).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hikaru Tsuchiya  

LAB HEAD: Yukiko Yoshida

PROVIDER: PXD053753 | Pride | 2024-08-06

REPOSITORIES: Pride

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20240122_Yoshida_Ub_PRM01_Nrf1_FBS2_01.raw Raw
20240122_Yoshida_Ub_PRM02_Nrf1_FBS2_02.raw Raw
20240122_Yoshida_Ub_PRM03_Nrf1_FBS2_03.raw Raw
20240122_Yoshida_Ub_PRM04_Nrf1_mut_01.raw Raw
20240122_Yoshida_Ub_PRM05_Nrf1_mut_02.raw Raw
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