Proteomics

Dataset Information

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Quantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice

ABSTRACT: Label free: The aim of project is to identify the proteins that may have a role in the inductive signal of mesenchymal cells.   Protein identification and quantitively compare the proteins from the following samples: 1- Exosomes purified from embryonic mesenchymal cells of tooth germ after overnight culture in 2D and 3D conditions 2- Secretome of embryonic mesenchymal cells of tooth germ after overnight 3D culture. 3- Embryonic mesenchymal cells from tooth germ- fresh frozen as pellet TMT Samples 10.5 and 14.5 are exosomes derived from immortalized cell line and different stages of tooth embryonic development. These sets of samples were seeded in well plate. The embryonic samples are a pool of 6 biological replicates. The aim is to identify the different proteins between these stages as well as the cell line.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Tooth Germ

SUBMITTER: Steven Lynham  

LAB HEAD: Prof. Paul Sharpe

PROVIDER: PXD053767 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
PR576_AB1_1_TMT6plex_Rep1_Rep3_MT_Low.msf Msf
PR576_AB1_1_TMT6plex_Rep2.raw Raw
PR576_AB1_1_TMT6plex_Rep3.raw Raw
PR576_AB2_1_3D_1.msf Msf
PR576_AB2_1_3D_1.raw Raw
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Publications

The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development.

Birjandi Anahid A AA   Sharpe Paul P  

Bioengineering (Basel, Switzerland) 20250121 2

Teeth develop from reciprocal signaling between inductive and receptive cells. The inductive signals for tooth development are initially in the epithelium of the developing branchial arch, but later shift to the underlying mesenchyme of a developing tooth germ. The inductive signals that are needed for tooth development have not yet been fully identified. Our lab previously provided a basis for bioengineering new teeth by separating the tooth germ's epithelial and mesenchyme cells into a single  ...[more]

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