Dataset Information

Erosive tooth wear and its association to risk, saliva and biofilm composition in Colombian adolescents

ABSTRACT: The aim of this study was to compare the protein composition of the acquired pellicle from adolescents with Erosive Tooth Wear (ETW) caries and sound. Calibrated examiners in BEWE Index and ICDAS-merged Epi criteria assessed ETW and caries in a sample of 454 systemically healthy adolescents aged 12 to 15 years old. From them, 30 subjects were selected for this study, presenting either Sound enamel (n=10, Total BEWE sum <8 and without extensive/cavitated caries lesions), ETW (n=10, Total BEWE sum ≥9 and without extensive/cavitated caries lesions) or Caries (n=10, Total BEWE sum <8 and with at least one Extensive caries lesion). Two hours formation acquired pellicle samples were taken from tooth surfaces (Buccal/Occlusal/Palatal/Lingual). Protein composition was analysed by Liquid Chromatography Tandem Mass Spectrometry. Using mean reporter ion values relative abundances of proteins were compared among the three groups to calculate for fold changes. Protein increases or decreases of 2-fold were reported (t-test, p<0.05). Gene Ontology (GO) of the included proteins was assigned. In the results of this project were found a total of 127 proteins in the acquired pellicle. The GO analyses showed that the majority of detected proteins were stress-response related. The ETW group disclosed up-regulated relative abundance of Antileucoprotease; Histatin and Prolactin-induced protein Hemoglobin subunits Alpha (HBA) and Beta (HBB) showed decreased relative abundances in the ETW and Caries groups when compared to the Sound group. As a conclusion, the acquired pellicle from individuals with ETW showed differences when compared to other dental conditions, with an increase in relative abundance of some stress response associated proteins in ETW and a decrease in proteins related to salivary protection against acid challenges.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tooth Enamel

DISEASE(S): Tooth Erosion

SUBMITTER: Steven Lynham

LAB HEAD: Prof. Stefania Martignon

PROVIDER: PXD053912 | Pride | 2026-01-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PR659_GP_VA_TMT11_1_11_1_Rep1_Rep3_HT_combined_High_RR.msf	Msf
	PR659_GP_VA_TMT11_1_11_1_Rep1_Rep3_HT_combined_High_RR.mzTab	Mztab
	PR659_GP_VA_TMT11_1_11_1_Rep1_Rep3_HT_combined_Low.msf	Msf
	PR659_GP_VA_TMT11_1_11_1_Rep1_Rep3_HT_combined_Low.mzTab	Mztab
	PR659_GP_VA_TMT11_1_Rep1_RR.raw	Raw

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Project description:Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. In recent years, biofilm development of S. oneidensis has been extensively studied because it is essential to reduce solid metals. As a special form of biofilm, however, pellicles are largely overlooked. The goal of this work was to understand requirements of S. oneidensis pellicle formation and the molecular basis of pellicle formation. We demonstrated that successful pellicle formation and survival was likely to require the threshold level of cell density and higher concentration of oxygen. Proteinase K and EDTA were potent pellicle disrupter. DNA microarray experiments were used to study the gene expression profile of young air–liquid interface pellicle relative to planktonic cells, which indicated that the air–liquid interface pellicle was more metabolically active than the planktonic cells. Most notably, consistently up-regulation of iron or heme uptake and transportation proteins was observed in the S. oneidensis MR-1 pellicle. However, neither the hmuT nor hugA heme transport mutant was defective in pellicle formation. An examination of the influence of several metal cations on the anti-pellicle activity of EDTA showed that Ca (II), Mn(II), Cu(II), and Zn(II) fully protected S. oneidensis MR-1 pellicle against EDTA treatment while additional of iron enabled the initiation of pellicle formation but maturation was significantly impaired. Collectively, iron was less important than other metals with respect to pellicle formation in S. oneidensis. A fresh colony grown overnight on a LB plate was used to inoculate 50 ml LB and incubated in a shaker (200 rpm) to an OD600 of 0.8 at the room temperature. This culture was then diluted 500-fold with fresh LB, resulting in the starting cultures. Aliquots of 30ml starting cultures were transferred to 50-ml Pyrex beakers and allowed to develop pellicles at the room temperature. When a complete but thin (young pellicle) at the interface were formed (about 30h hours), planktonic culture and pellicle were separated and applied to centrifugation at 8000 rpm for 3 min at room temperature. 3 parallel starting cultures were used and 3 samlpes of pellicle cells or planktonic cells were collected at 30h. RNA from the pellicle cells was fluorescently labeled with Cy3, and that from the planktonic was labeled with Cy5.

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Erosive tooth wear and its association to risk, saliva and biofilm composition in Colombian adolescents

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