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Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis

ABSTRACT: In mammals, chromosome-wide regulatory mechanisms ensure a balance of X-linked gene dosage between males (XY) and females (XX). In female cells, expression of genes from one of the two X-chromosomes is curtailed, with selective accumulation of Xist-RNA, Xist-associated proteins, specific histone modifications (eg. H3K27me3) and Barr body formation observed throughout interphase. Using chromosome flow-sorting, we show that during mitosis, Xist-associated proteins dissociate from inactive X (Xi) chromosomes, while high levels of H3K27me3 and increased compaction of the Xi relative to active X (Xa), are retained. Proteomic comparison of mitotic Xi and Xa revealed, unexpectedly, that components of Hbo1 and Msl/Mof histone acetyltransferase complexes co-enrich with Xa, while inhibitors of histone acetylation co-enrich with Xi. Furthermore, inhibition of Hbo1 or deletion of Msl/Mof components functionally abolishes mitotic differences in H3K27me3 marking and chromosome compaction. These data uncover critical roles for acetylation pathways in preserving X chromosome properties during mitosis.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell

SUBMITTER: Alex Montoya

LAB HEAD: Dr Amanda Fisher

PROVIDER: PXD054014 | Pride | 2025-05-23

REPOSITORIES: Pride

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			Action	DRS
	ChrXaXiXm_pool_1.raw	Raw
	ChrXaXiXm_pool_2.raw	Raw
	ChrXaXiXm_pool_3.raw	Raw
	PreB_SC_1.raw	Raw
	PreB_SC_2.raw	Raw

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