Proteomics

Dataset Information

Analysis of insulin-regulated phosphorylation of AFF4 with Akti or S6ki and S6K invitro kinase

ABSTRACT: Aff4 was recently identified as an insulin-regulated phosphoprotein. To investigate upstream kinases, we analysed immunoprecipitated Aff4 treated with our without insulin and treated with either an Akti or a P70S6Ki. These results showed that AFF4 is dependent on P70S6K and therefore an in vitro kinase assay was performed to validate direct phosphorylation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Benjamin Parker  

LAB HEAD: Benjamin Parker

PROVIDER: PXD054247 | Pride | 2025-12-16

REPOSITORIES: Pride

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Action DRS
221007_Eclipse_BenParker_BP-5158_A11.raw Raw
221007_Eclipse_BenParker_BP-5158_A12.raw Raw
221007_Eclipse_BenParker_BP-5158_B11.raw Raw
221007_Eclipse_BenParker_BP-5158_B12.raw Raw
221007_Eclipse_BenParker_BP-5158_C11.raw Raw
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