Proteomics

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Proteomic analysis of senescent translatome

ABSTRACT: To examine senescent translatome, we combined L-azidohomoalanine (AHA)-mediated labeling of newly synthesized proteins followed by click chemistry for biotin conjugation with the DiDBiT in proliferating and senescent cells. Senescence was induced using a stress-induced senescence model (bleomycin-induced senescence). We further examined the effect of EIF4H-S knockdown on the translatome using a shRNA targeting EIF4H-S.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Diploid Cell

DISEASE(S): Disease Free

SUBMITTER: Jong-Seo Kim  

LAB HEAD: Jong-Seo Kim

PROVIDER: PXD054317 | Pride | 2024-10-04

REPOSITORIES: Pride

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Tranlatome_AHAbiotin_ProAx_NAQ444_J100_08Sep2023_1.mzXML Mzxml
Tranlatome_AHAbiotin_ProAx_NAQ444_J100_08Sep2023_1.mzid.gz Mzid
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Tranlatome_AHAbiotin_Pro_1_NAQ444_J100_08Sep2023_1.mzXML Mzxml
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